QUANTITATIVE MICROTOMOGRAPHY OF BONE TRABECULAE

骨小梁的定量显微成像

• 批准号: 6653243
• 负责人: Michael J Flynn
• 金额: $ 10.73万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-25 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653243
• 关键词: X ray; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; bone imaging /visualization /scanning; bone regeneration; computed axial tomography; dogs; electromagnetic radiation; genetically modified animals; hormone therapy; laboratory mouse; method development; normal ossification; osteocytes; osteogenesis imperfecta; osteoporosis; ovariectomy; postmenopause; somatotropin; substantia spongiosa; synchrotrons; tomography

DESCRIPTION (provided by applicant): X-ray computed tomography of the spine and hip is an established method for quantitative bone mineral measurement that resolves elements with 1-millimeter size. For bone specimens, high-resolution laboratory computed tomography systems can describe cancellous bone architecture with resolved elements of about 50 micrometers. This is sufficient to describe bone volume, trabecular thickness and orientation, and connectivity. We have recently developed a new innovative method to perform quantitative computed tomography on an individual bone trabecula with resolved elements of 1 micrometer. Whole trabeculae are separated from cancellous bone specimens and scanned in a 250 micrometer diameter saline filled tube. A beam of x-rays from a synchrotron source is focused using a zone plate and used to make numerous measurements of radiation transmission. The measured data is then reconstructed to a 600 x 600 x 540 array of values having a spacing of 1/3 micrometers in the X, Y, and Z direction. High speed scanning devices will be used on this project to enable examination of 16 specimens per day. This method should be extremely valuable for evaluating the microscopic properties of bone tissue with respect to the mineralization of the matrix and porosity associated with osteocyte lacunae. To establish this value, four biomedical experiments are proposed for which genetic, pharmacologic, and biomechanic variables will be used to alter the bone tissue in animals. Prior experience indicates that these models should alter the mineralization and porosity at a microscopic scale.

描述（由申请人提供）：脊柱的 X 射线计算机断层扫描 髋关节是一种定量骨矿物质测量的既定方法 解析 1 毫米尺寸的元素。对于骨骼标本，高分辨率 实验室计算机断层扫描系统可以描述松质骨 具有约 50 微米分辨率元素的架构。这已经足够了 描述骨量、小梁厚度和方向，以及 连接性。我们最近开发了一种新的创新方法来执行 对单个骨小梁进行定量计算机断层扫描 1微米的元素。 将整个小梁从松质骨标本中分离出来并在扫描仪中进行扫描 250 微米直径的盐水填充管。来自同步加速器的 X 射线束 使用波带板聚焦源并用于进行多次测量 辐射传输。然后将测量数据重建为 600 x 600 x 540 值数组，X、Y 和 Z 方向的间距为 1/3 微米 方向。该项目将使用高速扫描设备来实现 每天检查16个样本。 该方法对于评估微观结构非常有价值 骨组织与基质矿化有关的特性和 与骨细胞腔隙相关的孔隙度。为了确定这个值，需要四个 提出了生物医学实验，其中遗传、药理学和 生物力学变量将用于改变动物的骨组织。事先的 经验表明这些模型应该改变矿化和 微观尺度的孔隙率。