NOVEL X-RAY TECHNOLOGY FOR DEGENERATIVE JOINT DISEASE

用于治疗退行性关节疾病的新型 X 射线技术

基本信息

批准号：
6620957
负责人：
Carol Muehleman
金额：
$ 69.66万
依托单位：
RUSH UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-06-01 至 2007-05-31
项目状态：
已结题

项目摘要

We propose a program to integrate biomedical (Rush Medical College) with bioengineering (Illinois Institute of Technology and Massachusetts Institute of Technology) approaches to test and refine a novel X-ray technology for the diagnosis of joint pathology, particularly osteoarthritis. This technology may aid in the development of disease modifying agents and treatment strategies for the prevention and treatment of joint diseases. This project utilizes a novel synchrotron x-ray technique called diffraction enhanced imaging (DEI) which derives dramatic gains in contrast over conventional radiographs by exploiting x-ray refraction and scatter rejection (extinction) in addition to the usual absorption of conventional radiography. This technique, originally developed for mammary carcinoma imaging analyzes soft tissue as high contrast images with very high (greater than 0.05mm) spatial resolution. Although the synchrotron is currently used for DE imaging, the technique is not, in principle, tied to it. We have already shown that DEI is capable of imaging normal and degenerated articular cartilage of synovial joints showing features unique to this type of imaging using exposure times comparable to those of ordinary radiography. Beginning in the first year, we will interpret the cartilage and bone data obtained through our DEI methodologies by using the biological profiles of the matrix components as garnered through morphologic, biochemical and biophysical analysis. Some of the features observed in the DE images are not immediately explainable in molecular, chemical or structural terms. By using a unique integrated experimental approach, correlating biochemical and morphological tissue profiles with DE images, we hope to refine the overall DEI system for the detection of joint disease and, potentially, for other pathologies. We will image human and animal synovial joints and begin the refinement of the imaging technique for the optimal identification of early cartilage lesions. Animal models of cartilage degeneration will be used particularly for DE imaging of cartilage prior to visible signs of degeneration. Beginning in year two, we will image human cartilage that has been biomechanically damaged under controlled conditions for observation through DE imaging. We will also begin developing new DEI methodologies to produce images conveying more comprehensive information about the properties of the cartilage tissue, first for planar and then for 3D computed tomography. Throughout years one through five of the proposed project, there will be an iterative process of comparing biological analytical data with imaging data for the refinement of the DEI technique for joint tissues. Our long-term goal is to identify and localize initial phases of cartilage degeneration and follow their progression with the ultimate aim of monitoring disease progression and therapeutic interventions.

我们提出了一项计划，将生物医学（拉什医学院）与生物工程（伊利诺伊理工学院和麻省理工学院）方法相结合，以测试和完善用于诊断关节病理学（特别是骨关节炎）的新型 X 射线技术。该技术可能有助于开发疾病调节剂和预防和治疗关节疾病的治疗策略。该项目采用了一种称为衍射增强成像 (DEI) 的新型同步加速器 X 射线技术，除了传统放射线照相的通常吸收之外，还利用 X 射线折射和散射抑制（消光），与传统放射线照相相比，获得了显着的对比度增益。这项技术最初是为乳腺癌成像而开发的，可将软组织分析为具有非常高（大于 0.05 毫米）空间分辨率的高对比度图像。尽管同步加速器目前用于 DE 成像，但原则上该技术并不与其相关。我们已经证明，DEI 能够对滑膜关节的正常和退化关节软骨进行成像，并使用与普通放射线照相相当的曝光时间显示出此类成像的独特特征。从第一年开始，我们将使用通过形态学、生物化学和生物物理分析获得的基质成分的生物特征来解释通过我们的 DEI 方法获得的软骨和骨骼数据。 DE 图像中观察到的一些特征无法立即用分子、化学或结构术语进行解释。通过使用独特的综合实验方法，将生化和形态组织概况与 DE 图像相关联，我们希望完善用于检测关节疾病以及其他病理的整体 DEI 系统。我们将对人类和动物滑膜关节进行成像，并开始改进成像技术，以最佳地识别早期软骨病变。软骨退变的动物模型将特别用于在出现明显退变迹象之前对软骨进行 DE 成像。从第二年开始，我们将在受控条件下对生物力学受损的人类软骨进行成像，以便通过 DE 成像进行观察。我们还将开始开发新的 DEI 方法，以生成能够传达有关软骨组织特性的更全面信息的图像，首先用于平面，然后用于 3D 计算机断层扫描。在拟议项目的第一年到第五年中，将有一个迭代过程，将生物分析数据与成像数据进行比较，以改进关节组织的 DEI 技术。我们的长期目标是识别和定位软骨退化的初始阶段，并跟踪其进展，最终目的是监测疾病进展和治疗干预。