Laser Capture Microdissection (LCM) System

激光捕获显微切割 (LCM) 系统

• 批准号: 6731544
• 负责人: ROBERT H. HELFERT
• 金额: $ 12.45万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY SCH OF MED
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731544
• 关键词: biomedical equipment purchase; cell sorting; cell type; gene expression; laser capture microdissection; polymerase chain reaction; protein structure

DESCRIPTION (provided by applicant): An arsenal of potent assay methods is available for the analyses of genes and the products of their expression. These new tools require highly purified DNA, RNA or protein samples. To most effectively study disease processes, investigators should ideally obtain their samples from a precisely defined organ substructure or a homogeneous population of cells isolated from organs or tissues that are usually quite heterogeneous in cellular makeup. This task may be difficult if the cells of interest constitute a low percentage of the total tissue volume. Laser capture microdissection (LCM) allows the identification of specific sub-structures or cell types and their precise removal from the tissue of origin without damage. LCM offers important advantages over other techniques for cell purification, particularly with respect to precision, speed, simplicity, and archiving capabilities. The acquisition of a Pix Cell lie LCM System at the SIU School of Medicine (SIUSM) will allow our NIH-funded faculty to collect highly defined samples for subsequent genomic analysis. Although our faculty is already experienced at conventional PCR, quantitative real-time PCR (QRT-PCR) offers numerous advantages. Integration of QRT-PCR capability with the Pix Cell lIe LCM system will allow participants to accurately quantify gene expression and to link changes in expression to specific cell populations. As an accessory to the LCM, we are therefore requesting four Smart Cycler II 16-site, add-on blocks. These modules will be used to expand our existing but very limited QRT-PCR capabilities to support a multi-user environment SIUSM and its core Research Imaging Facility will provide the necessary infrastructure and financial support to keep the requested equipment in excellent working order and to promote optimal use by participating faculty. The requested LCM and complementary QRT-PCR instrumentation will allow participating faculty to acquire additional high quality data in support of their current NIH-funded research programs (NHLBI, NINDS, NCRR, NIA, NCI, NICHD, NIDCD). The precise and definitive results afforded by LCM and QRT-PCR are not possible or feasible with existing technology at the SIUSM. The availability of LCM with integrated QRT-PCR as a core facility will greatly strengthen the on-going strong NIH-funded research programs of our faculty.

描述（由申请人提供）：可用于分析基因及其表达产物的有效测定方法的库。这些新工具需要高度纯化的DNA，RNA或蛋白质样品。对于最有效地研究疾病过程，研究人员应理想地从精确定义的器官下结构或从通常在细胞构成中非常异构的器官或组织中分离出的均质细胞中获取样品。如果感兴趣的细胞占总组织量的较低比例，则此任务可能很困难。 激光捕获显微解剖（LCM）允许鉴定特定的子结构或细胞类型及其从原始组织中的精确去除而不会损坏。 LCM比其他细胞纯化具有重要优势，尤其是在精确，速度，简单和归档功能方面。在SIU医学院（SIUSM）中获取PIX细胞Lie LCM系统将允许我们的NIH资助的教师收集高度定义的样本，以进行后续的基因组分析。尽管我们的教师已经在常规PCR中经验丰富，但定量实时PCR（QRT-PCR）具有许多优势。 QRT-PCR能力与Pix细胞谎言LCM系统的整合将使参与者能够准确量化基因表达并将表达变化链接到特定细胞群体。因此，作为LCM的配件，我们要求四个Smart Cycler II 16地点附加块。这些模块将用于扩展我们现有但非常有限的QRT-PCR功能，以支持多用户环境 Siusm及其核心研究成像设施将提供必要的基础设施和财务支持，以使所需的设备保持出色的工作状态，并通过参与教师促进最佳使用。请求的LCM和补充QRT-PCR仪器将允许参与的教师获取更多的高质量数据，以支持其当前的NIH资助研究计划（NHLBI，NINDS，NCRR，NCRR，NIA，NIA，NCI，NCI，NICHD，NICHD，NIDCD）。 LCM和QRT-PCR提供的确切和确定的结果是SIUISM的现有技术是不可能或可行的。 LCM以QRT-PCR为核心设施的LCM的可用性将大大加强我们教职员工的持续强大的NIH资助研究计划。