Transmission Electron Microscope

透射电子显微镜

基本信息

批准号：
6813400
负责人：
Timothy S Baker
金额：
$ 200万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-07-15 至 2005-07-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Molecular electron cryo-microscopy (cryoEM) is at the same stage of development today as macromolecular x-ray crystallography was in 1980. We are entering an exponential growth phase of this technology accompanied by significant expansion in the number of practitioners and applications. Publications describing the utilization molecular cryoEM in combination with crystallography have grown dramatically and they have revealed exceptionally interesting features of mechanistic biology. Molecular cryoEM fills a unique niche between high resolution cytological EM and crystallography. Examining specimens not immobilized in a crystal lattice has allowed the characterization of dynamic features of macromolecular assemblies impossible to characterize by crystallography and to determine sub nanometer structures of species too large or too heterogeneous to be crystallized. A remarkably powerful strategy has been to determine high-resolution structures of the components of a macromolecular complex by crystallography or NMR followed by the construction of a pseudo atomic resolution model based on the cryoEM reconstruction of the intact organization. At resolutions currently achievable with high voltage FEG instruments, the precision of this modeling into cryoEM density is exceptionally high. This application requests partial support from NIH for a 300KV electron microscope equipped with a field emission gun, a helium cryo stage and a 4k by 4k slow scan CCD. This instrument will fill a gap that exists on the Torrey Pines Mesa in the atoms to cells continuum of structural studies that is the backbone of the world-class biological science produced by the participating institutions; University of California, San Diego; The Burnham Institute and The Scripps Research Institute. Projects proposed for high resolution work with this instrument include molecular motors, membrane proteins and their interactions, complexes controlling intercellular communication and virus assembly and maturation. In addition, software and hardware developed for high throughput EM data collection will be implemented on the requested microscope as it is perfected. These are all well funded programs that have benefited from moderate resolution studies and will immediately utilize the new technology when it is available. We anticipate that a broad range of projects from the Torrey Pines Mesa will find this technology useful as investigators not currently directly involved with molecular cryoEM are introduced to results emerging from its use.

描述（由申请人提供）：分子电子冷冻微观镜（Cryoem）在当今的发育阶段与大分子X射线晶体学的同一阶段。我们正在1980年。我们正在进入该技术的指数增长阶段，并伴随着从业者和应用程序的数量大量扩展。描述使用分子冷冻与晶体学结合使用的出版物已经显着增长，它们揭示了机械生物学的异常有趣的特征。分子冷冻填充了高分辨率细胞学EM和晶体学之间的独特利基。检查未固定在晶体晶格中的标本已使大分子组件的动态特征表征不可能通过晶体学表征，并确定太大或太大的物种的亚纳米结构，无法结晶。一种非常有力的策略是通过晶体学或NMR确定大分子复合物组件的高分辨率结构，然后基于完整组织的冷冻重建建造伪原子分辨率模型。在目前使用高压FEG仪器可以实现的分辨率下，该模型中的冷冻密度的精度异常高。该申请要求NIH的部分支持，该支持配备了田间发射枪，氦气冷冻阶段和4K乘4K慢速扫描CCD。该仪器将填补原子中托里松树膜上存在的空白，与参与机构产生的世界一流生物科学的骨干结构研究连续。加利福尼亚大学圣地亚哥；伯纳姆研究所和斯克里普斯研究所。该仪器提供高分辨率工作的项目包括分子电机，膜蛋白及其相互作用，控制细胞间通信以及病毒组装和成熟的复合物。此外，为高吞吐量EM数据收集而开发的软件和硬件将在所需的显微镜上实现。这些都是资金良好的计划，从中等分辨率的研究中受益，并将在可用时立即利用该技术。我们预计Torrey Pines Mesa的广泛项目将发现这项技术有用，因为目前尚未直接参与分子冷冻的调查人员会从其使用中引起结果。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Enhanced magnetic resonance contrast of Fe₃O₄ nanoparticles trapped in a porous silicon nanoparticle host.

DOI：
10.1002/adma.201101877
发表时间：
2011-09-22
期刊：
ADVANCED MATERIALS
影响因子：
29.4
作者：
Kinsella, Joseph M.;Ananda, Shalini;Andrew, Jennifer S.;Grondek, Joel F.;Chien, Miao-Ping;Scadeng, Miriam;Gianneschi, Nathan C.;Ruoslahti, Erkki;Sailor, Michael J.
通讯作者：
Sailor, Michael J.

Three-dimensional reconstructions of the bacteriophage CUS-3 virion reveal a conserved coat protein I-domain but a distinct tailspike receptor-binding domain.

噬菌体 CUS-3 病毒粒子的三维重建揭示了保守的外壳蛋白 I 结构域和独特的尾刺受体结合结构域。

DOI：
10.1016/j.virol.2014.06.017
发表时间：
2014
期刊：
Virology
影响因子：
3.7
作者：
Parent,KristinN;Tang,Jinghua;Cardone,Giovanni;Gilcrease,EddieB;Janssen,MandyE;Olson,NormanH;Casjens,SherwoodR;Baker,TimothyS
通讯作者：
Baker,TimothyS

X-ray computed tomography imaging of breast cancer by using targeted peptide-labeled bismuth sulfide nanoparticles.

DOI：
10.1002/anie.201104507
发表时间：
2011-12-16
期刊：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
影响因子：
16.6
作者：
Kinsella, Joseph M.;Jimenez, Rebecca E.;Karmali, Priya P.;Rush, Anthony M.;Kotamraju, V. Ramana;Gianneschi, Nathan C.;Ruoslahti, Erkki;Stupack, Dwayne;Sailor, Michael J.
通讯作者：
Sailor, Michael J.

Self-assembly of coherently dynamic, auxetic, two-dimensional protein crystals.