CORE--RESEARCH CYTOGENETICS

核心--研究细胞遗传学

• 批准号: 6652221
• 负责人: Joseph R. Testa
• 金额: $ 19.62万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6652221
• 关键词: CORE; RESEARCH; CYTOGENETICS

Description: (Applicant's Description) The Research Cytogenetics Core Facility (RCCF) assists funded Fox Chase Cancer Center (FCCC) investigators in research projects that require karyotypic and molecular cytogenetic analyses of mammalian cells. Since its inception in July 1995, the RCCF has been used by 27 peer-review funded investigators in 11 Programs from all three Divisions. In addition to routine processing of chromosomes from tumor tissues and cell lines, the Facility performs special staining of metaphase preparations and carries out detailed analyses of banded chromosomes. The RCCF maps the precise chromosomal location of genes by fluorescence in situ hybridization, using DNA probes provided by the investigator. Furthermore, the RCCF performs comparative genomic hybridization analyses of tumor and drug-resistant cell lines. The Facility also provides FCCC investigators with access to a CCD camera for digitized immunofluorescence of antibody staining. The work performed has included 214 tests, as follows: chromosome/karyotype analyses (89 tests), CGH analyses (17), FISH mapping (23), digitized immunofluorescence of cytological preparations (71), microdissection of cytological preparations (10), and image preparation/print processing (4). The Facility is expected to be used by 10-12 investigators per year in years 2000-2005, with a total of approximately 40 tests/year. The RCCF is well equipped for research cytogenetics. Specific equipment includes a Zeiss Axiophot photomicroscope with fluorescence optics (including an attached cooled CCD camera operated by a Macintosh based workstation for digitized imaging), a Nikon Microphot brightfield photomicroscope with an attached CCD camera and automated karyotype workstation, a Zeiss inverted photomicroscope with affixed Eppendorf micromanipulator for microdissection, photographic enlarger, a rapid print processing system, and a color printer.

描述：（申请人的描述）研究细胞遗传学核心 设施 (RCCF) 协助福克斯蔡斯癌症中心 (FCCC) 的研究人员 需要核型和分子细胞遗传学分析的研究项目 哺乳动物细胞。自 1995 年 7 月成立以来，RCCF 一直使用 由 27 名同行评审资助的研究人员在所有三个项目的 11 个项目中进行 部门。除了对肿瘤染色体进行常规处理外 组织和细胞系，该设施对中期进行特殊染色 准备并对带状染色体进行详细分析。这 RCCF 通过荧光原位绘制基因的精确染色体位置图 杂交，使用研究者提供的DNA探针。此外， RCCF 对肿瘤和肿瘤进行比较基因组杂交分析 耐药细胞系。该设施还为 FCCC 调查人员提供 使用 CCD 相机进行抗体染色的数字化免疫荧光。 所做的工作包括 214 项测试，如下：染色体/核型 分析（89 项测试）、CGH 分析 (17)、FISH 绘图 (23)、数字化 细胞学制剂的免疫荧光 (71)、显微解剖 细胞学准备 (10) 和图像准备/打印处理 (4)。 预计数年内每年将有 10-12 名研究人员使用该设施 2000-2005年，每年总共约40次测试。 RCCF 还不错 配备用于研究细胞遗传学。具体设备包括蔡司 带荧光光学器件的 Axiophot 显微显微镜（包括附加的 由基于 Macintosh 的工作站操作的冷却 CCD 相机，用于数字化 成像），带有 CCD 的 Nikon Microphoto 明场显微镜 相机和自动核型工作站、蔡司倒置显微镜 带有固定的 Eppendorf 显微操作器，用于显微解剖、照相 放大机、快速打印处理系统和彩色打印机。