XRAY ROTATION CAMERA STUDIES OF SULFITE OXIDASE

亚硫酸氧化酶的 X 射线旋转摄像机研究

• 批准号: 6586604
• 负责人: JOHN H ENEMARK
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586604
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; enzymes; proteins; radiography; structural biology

Small plate-shaped crystals of sulfite oxidase, a molybdenum containing enzyme, have been obtained under two different pH conditions. Sulfite oxidase is a molybdopterin enzyme, of which there are three families: the xanthine oxidase family; the DMSO reductase family; and the sulfite oxidase family. Crystal structures of enzymes in the first two families have recently been solved, however, there is no structure available from the sulfite oxidase family. Preliminary experiments have shown that synchrotron radiation sources and the rotation camera are required to obtain x-ray diffraction data from these small plate-shaped crystals of sulfite oxidase.

亚硫酸氧化酶（一种钼）的小片状晶体 含有酶，在两种不同pH值下获得 状况。 亚硫酸盐氧化酶是一种钼蝶呤酶，其中有 是三个家族：黄嘌呤氧化酶家族；二甲基亚砜还原酶 家庭;和亚硫酸盐氧化酶家族。 酶的晶体结构 前两个家庭最近已得到解决，但是，还有 亚硫酸盐氧化酶家族没有可用的结构。 初步的 实验表明，同步辐射源和 需要旋转相机来获取 X 射线衍射数据 这些亚硫酸氧化酶的小板状晶体。