Core: Array

核心：数组

• 批准号: 6678762
• 负责人: Eleen Mulvihill
• 金额: $ 40.52万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6678762
• 关键词: RNA; atherosclerosis; atherosclerotic plaque; biomarker; biomedical facility; confocal scanning microscopy; gene expression; immunocytochemistry; inflammation; laser capture microdissection; lymphocyte; magnetic resonance imaging; messenger RNA; microarray technology; monocyte; northern blottings; pathologic process; polymerase chain reaction; technology /technique development; tissue /cell culture

The Array Core hopes to accumulate microarray expression data that explores the view that inflammatory mechanisms are fundamental to plaque progression and plaque rupture. While this view is widely held and probably valid in the early stages of disease {Blake CR 2001;5962;4893 }, direct evidence for involvement in plaque progression in man, as opposed to fatty streak initiation in mice, remains elusive. The goal of Core A is to use the tissue and blood samples collected in the Projects to derive morphological and expression data relevant to the exploration of this hypothesis. A large part of this effort will be focused on determining if lymphocytes and monocytes can be used as sensor cells, that is, as cell types likely to report the presence of pathological states. The core will add to the effort of the Projects by exploring the possibility that analysis of mRNA content in peripheral blood monocytes and leukocytes could provide a "biomarker" for relevant states of inflammation that correlate with plaque progression. The mode for analysis of these cells is to use expression arrays to test specific hypotheses, i.e. does array based analysis have the ability to identify and cluster patients with distinct behavior of their plaques as shown by MRI, by genetic background, by a specific clinical parameter or by the presence or absence of a specific cell subset or subset specific activity or behavior. Use of expression arrays to categorize any disease, except perhaps for cancer, is still a frontier. The Core will work at this frontier and we hope our progress will give new insights into the mechanisms leading to lesion progression.

Array Core 希望积累微阵列表达数据，探索炎症机制是斑块进展和斑块破裂的基础这一观点。虽然这种观点被广泛持有，并且在疾病的早期阶段可能是有效的{Blake CR 2001;5962;4893}，但与小鼠脂肪纹起始相反，涉及人类斑块进展的直接证据仍然难以捉摸。核心 A 的目标是使用项目中收集的组织和血液样本来获取与探索该假设相关的形态和表达数据。这项工作的很大一部分将集中于确定淋巴细胞和单核细胞是否可以用作传感器细胞，即可能报告存在的细胞类型 病理状态。该核心将通过探索外周血单核细胞和白细胞中 mRNA 含量的分析可以为与斑块进展相关的炎症状态提供“生物标志物”的可能性来增加该项目的努力。这些细胞的分析模式是使用表达阵列来测试特定假设，即基于阵列的分析是否能够识别和聚类具有 MRI 显示的斑块不同行为的患者、遗传背景、特定临床参数或通过特定细胞亚群或亚群特定活动或行为的存在或不存在。使用表达阵列对任何疾病（癌症除外）进行分类仍然是一个前沿领域。这 Core 将致力于这一前沿领域，我们希望我们的进展能够为导致病变进展的机制提供新的见解。