The role of VEGF isoforms in tumor angiogenesis

VEGF亚型在肿瘤血管生成中的作用

• 批准号: 6912661
• 负责人: ERIC B FINKELSTEIN
• 金额: $ 4.99万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-10 至 2007-06-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6912661
• 关键词: SDS polyacrylamide gel electrophoresis; angiogenesis; cell differentiation; connective tissue cells; disease /disorder model; embryo /fetus cell /tissue; fibroblasts; gene expression; genetically modified animals; growth factor receptors; immunoprecipitation; laboratory mouse; lung neoplasms; neoplasm /cancer blood supply; neoplastic cell; neoplastic growth; pancreatic islet neoplasm; postdoctoral investigator; protein isoforms; protein localization; proteolysis; transfection; vascular endothelial growth factors

DESCRIPTION (provided by applicant): The overall goal of this research proposal is to investigate the functional significance of vascular endothelial growth factor (VEGF) isoforms during tumor growth and angiogenesis using mouse models. There is growing evidence to support differential functions of VEGF isoforms. My proposed studies will use a spontaneous tumorigenesis model, as well as tumor implant models in which the tumor cells and/or host stromal cells express specific VEGF isoforms. In the spontaneous tumor model, I will determine the function of VEGF isoforms expressed by tumor and stroma cells in the sequence of events leading from normal cells to solid tumors. In the tumor implant models, I will determine the relative contribution of VEGF isoforms expressed in the tumor or the surrounding stromal cells. Finally, potential mechanisms of differential VEGF isoform action will be assessed.

描述（由申请人提供）：该研究建议的总体目标是研究使用小鼠模型在肿瘤生长和血管生成期间血管内皮生长因子（VEGF）同工型的功能意义。越来越多的证据支持VEGF同工型的差异功能。我提出的研究将使用自发的肿瘤发生模型，以及肿瘤植入物模型，其中肿瘤细胞和/或宿主基质细胞表达特定的VEGF同工型。在自发的肿瘤模型中，我将确定由肿瘤和基质细胞表达的VEGF同工型在从正常细胞到实体瘤的事件序列中表达的。在肿瘤植入物模型中，我将确定在肿瘤或周围基质细胞中表达的VEGF同工型的相对贡献。最后，将评估差异VEGF同工型作用的潜在机制。