Radioprotective Potential of Ginseng

人参的辐射防护潜​​力

• 批准号: 6984579
• 负责人: TUNG-KWANG LEE
• 金额: $ 16.03万
• 依托单位: EAST CAROLINA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6984579
• 关键词: DNA damage; alternative medicine; angiosperms; antioxidants; apoptosis; bioterrorism /chemical warfare; chemoprevention; clinical research; flow cytometry; free radical oxygen; human subject; human therapy evaluation; lymphocyte; medicinal plants; occupational hazard; oxidative stress; radiation hazard; radiobiology; radioprotective agents

DESCRIPTION (provided by applicant): The widespread use of radiation in such areas as medicine, agriculture and industry, along with the elevating threat of nuclear terrorism, has made radiation-induced normal tissue damage an increasing focus of concern for both civilians and the military. Thus, the need to identify radioprotectors that effectively reduce the biological effects of radiation, but lack the unwanted side effects associated with current radioprotectors, has taken on increasing urgency. In this regard, we have recently reported a dose-dependent relationship between Asian ginseng extracts and radioprotection in human peripheral blood lymphocytes (PBL) ex vivo. We believe this ex vivo radioprotection is indicative of ginseng's potential to function as an effective radioprotector in vivo. Our specific hypothesis is that ginseng possesses the potential to be an effective radioprotector in vivo, and, since free radicals play such an important role in radiation-induced damage, the mechanism underlying this radioprotection is linked, either directly or indirectly, to its antioxidative ability. Using a standardized North American Ginseng extract (NAGE) as our radioprotective agent, the specific aims are to: 1. Define the radioprotective potential of NAGE using our ex vivo PBL model by determining the effect of NAGE on PBL, before and after exposure to radiation, based on the degree of DNA damage; 2. Identify whether the mechanism underlying NAGE's radioprotection is correlated with its antioxidative action by assessing the cellular oxidative/antioxidative status in PBL before and after the radiation exposure and/or NAGE administration; and 3. Determine whether oral, daily supplementation of NAGE to healthy human subjects results in radioprotection of their PBL by comparing, both before and after administration of NAGE, the radiation-induced DNA damage in PBL drawn from subjects and exposed ex vivo to a range of radiation doses (0-2 Gy). Accomplishing these aims will provide an essential prerequisite to the development of NAGE as an effective radioprotector.

描述（由申请人提供）：在医学，农业和工业等地区广泛使用辐射，以及核恐怖主义的提升威胁，使辐射引起的正常组织破坏成为平民和军队的越来越关注的关注点。因此，鉴定有效减少辐射生物学作用但缺乏与当前辐射保护剂相关的不良副作用的辐射保护剂的需求已经对增加紧迫性。在这方面，我们最近报道了亚洲人参提取物与人类外周血淋巴细胞（PBL）EX VIVO中的剂量依赖性关系。我们认为，这种离体放射保护表明人参有效地充当体内有效的辐射保护剂的潜力。我们的具体假设是，人参具有在体内有效辐射保护剂的潜力，并且由于自由基在辐射诱导的损伤中起着如此重要的作用，因此该辐射保护的机制直接或间接地将其与其抗氧化能力联系起来。 。使用标准化的北美人参提取物（NAGE）作为我们的放射保护剂，具体目的是：1。使用我们的Ex Vivo PBL模型来定义Nage的辐射保护潜力，通过确定Nage对PBL的影响，暴露于辐射之前和之后，基于DNA损伤程度； 2.通过评估辐射暴露和/或纳奇给药之前和之后，确定NAGE辐射保护的基础机制是否与其抗氧化作用相关。和3。确定口服，每天补充Nage对健康的人类受试者是否通过比较NAGE给药前后，辐射引起的PBL造成的DNA损伤从受试者中抽出的PBL并在范围辐射剂量（0-2 Gy）。实现这些目标将为Nage作为有效的放射线保护剂的发展提供必不可少的先决条件。