Electrospun Collagen Type II: Cartilage Feasibilty

静电纺丝 II 型胶原蛋白：软骨可行性

• 批准号: 6945376
• 负责人: Gary L Bowlin
• 金额: $ 21.27万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6945376
• 关键词: SDS polyacrylamide gel electrophoresis; animal tissue; articular cartilage; bioengineering /biomedical engineering; biomaterial development /preparation; biomechanics; cartilage metabolism; cell line; cell proliferation; chondrocytes; clinical research; collagen; extracellular matrix; human tissue; mucopolysaccharides; nanotechnology; osteoarthritis; tissue /cell culture; tissue engineering; tissue support frame; western blottings

DESCRIPTION (provided by applicant): Osteoarthritis (OA), also known as degenerative joint disease, is one of the most common types of arthritis and is characterized, or associated, with the breakdown of the extracellular matrix (ECM) of articular cartilage. This deterioration of the articular cartilage ECM leads to bone exposure and subsequent bone-on-bone rubbing and associated inflammation that causes pain and loss of movement in the joint. According to the Arthritis Foundation, OA affects an estimated 21 million people in the United States alone with up to 80% of those individuals reporting some limitation in movement or activity. This costs the United Sates economy an estimated $65 billion per year in direct expenses and lost wages/production. Collagen is the natural scaffolding found in all tissues and has been explored extensively for use as a tissue-engineering scaffold with limited success. In this feasibility study, the electrospinning of collagen type II and subsequent chondrocyte seeding will be investigated for potential use in cartilage tissue engineering (Patents Pending). A preliminary of study electrospun collagen type II demonstrated that collagen type II could be electrospun to form non-woven fibrous mats composed of fibers that ranged from 110 nm to 1.8 microns in diameter. The preliminary chondrocyte seeding demonstrated that electrospun collagen type II scaffolds support cell growth and are readily infiltrated. Furthermore, detailed, feasibility studies are now proposed herein. Specific Aim 1 will be collagen type II preliminary processing from bovine articular cartilage including extraction of collagen and purification. Specific Aim 2 will be to electrospin various structures of randomly orientated collagen type II and create a diversity of physical and mechanical characteristics. Specific Aim 3 will characterize the biomechanical/structural properties of the electrospun collagen type II constructs (dry and hydrated) by tensile testing of the bulk material as well as measuring fiber diameter, pore dimensions, scaffold permeability and porosity. Specific Aim 4 will evaluate the collagen type II composition and overall structural properties (fiber diameter and pore size) on the chondrocytes response and overall tissue development after optimum cell seeding and 2, 4 and 6 week interactions with the collagen type II.

描述（由申请人提供）： 骨关节炎（OA），也称为退化性关节疾病，是最常见的关节炎类型之一，与关节软骨的细胞外基质（ECM）分解相关或相关。关节软骨ECM的这种恶化导致骨骼暴露和随后的骨头摩擦以及相关的炎症，从而导致关节疼痛和运动丧失。根据关节炎基金会的说法，OA仅影响美国的2100万人，其中80％的人报告了运动或活动的一定限制。这使联合国经济估计每年有650亿美元的直接支出和工资/产量损失。胶原蛋白是在所有组织中发现的自然脚手架，并且已广泛探索作为组织工程脚手架，成功有限。在这项可行性研究中，将研究胶原蛋白II型和随后的软骨细胞播种的静电纺丝，以便在软骨组织工程中使用（专利待处理）。研究的初步电纺胶原II型II类表明，II型胶原蛋白可以是静电传播的，形成由纤维组成的非织纤维垫，该纤维的直径范围为110 nm至1.8微米。初步的软骨细胞播种表明，电纺胶原II型支架支持细胞生长，并且很容易浸润。此外，现在提出了详细的可行性研究。具体目标1将是牛胶原型胶原蛋白的初步加工，包括牛关节软骨，包括提取胶原蛋白和纯化。具体目标2将是静电素的各种结构，这些结构随机定向胶原蛋白II型，并创造出多种物理和机械特征。特定的目标3将表征通过散装材料的拉伸测试以及测量纤维直径，孔尺寸，支架的渗透率和孔隙率，通过拉伸测试来表征电纺胶原II型II型构建体（干和水合）的生物力学/结构特性。特定的目标4将评估在最佳细胞播种后的软骨细胞反应和整体组织发育以及与胶原蛋白II型的2、4和6周相互作用后，评估软骨细胞反应和整体组织发育的胶原蛋白II型组成和整体结构特性（纤维直径和孔径）。