Magnetic Levitation of Osteoblasts

成骨细胞的磁悬浮

• 批准号: 7037343
• 负责人: BRUCE E HAMMER
• 金额: $ 21.71万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7037343
• 关键词: Magnetic; Levitation; Osteoblasts

DESCRIPTION (provided by applicant): The goal of the proposed research is two-fold. First, does a time-invariant magnetic field, between 1.5 Tesla and 17 Tesla, affect gene expression and cell differentiation? Second, is magnetic levitation a suitable ground-based alternative for space-based orbital freefall experiments? For the levitation studies we plan to monitor gene expression via microarray analysis and osteoblast differentiation by quantitative real-time PCR. The murine calvarial osteoblast cell line, MC3T3-E1, is the model biological system for these experiments. Magnetic levitation occurs when a magnetic force counterbalances a gravitational force. Placing a biological (diamagnetic) object in a strong magnetic field and a strong magnetic field gradient creates a magnetic force on the system. A magnetic force is the only means to reduce the net gravitational force on a system to less than 1-g. Osteoblast cells have a demonstrated sensitivity to gravitational loading conditions. These cells will be cultured in a unique 17 Tesla/50 mm warm bore superconductive magnet to study the effect of a net 'gravitation force varying from 0-g through 2-g on gene expression and osteoblast biochemical markers. The magnet is capable of sustaining continuous levitation for weeks at a time. Monitoring bone osteoblast differentiation and function under conditions where net the gravitational force is a variable, represents a unique venue for understanding the effect of gravitational forces on bone growth/resorption. This is a new area of scientific exploration that has not been explored because of limited access to magnet systems capable of magnetic levitation. Furthermore, with this instrument it is now possible to study biological function at 0.38 g and 0.167 g, thus providing ground-based simulations of the gravitational forces experienced on the surface of mars and the moon, respectively.

描述（由申请人提供）：拟议的研究的目标是两个方面。首先，在1.5特斯拉和17特斯拉之间的时间不变的磁场会影响基因表达和细胞分化吗？其次，磁悬浮是否是空间基轨道自由落体实验的合适地面替代方案？对于悬浮研究，我们计划通过微阵列分析和成骨细胞通过定量实时PCR来监测基因表达。鼠钙质成骨细胞系MC3T3-E1是这些实验的模型生物系统。当磁力平衡引力力时，就会发生磁悬浮。将生物学（Diamagnetic）物体放置在强磁场中，并且强磁场梯度在系统上产生磁力。磁力是将系统上的净重力减少到小于1克的唯一手段。成骨细胞对重力载荷条件具有敏感性。这些细胞将在独特的17特斯拉/50 mm温暖的钻孔中培养，以研究从0-G到2-G的净引力对基因表达和成骨细胞生物化学标记的影响。磁铁能够一次维持连续悬浮数周的连续悬浮。监测净骨成骨细胞的分化和功能在净重力是变量的条件下，代表了理解重力对骨骼生长/吸收的影响的独特场所。这是一个科学探索的新领域，由于对能够磁性悬浮的磁铁系统的访问有限，因此尚未探索。此外，借助该仪器，现在可以在0.38 g和0.167 g下研究生物学功能，从而分别对MARS和MOON表面经历的引力基于地面的模拟。