GLYCOSYL COMPOSITION ANALYSIS

糖基成分分析

• 批准号: 6653594
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653594
• 关键词: bacteria; bioengineering /biomedical engineering; biomedical resource; carbohydrates; environmental health; spectrometry

Glycosyl composition analysis was performed on a bacterial exopolysaccharide from Pseudomonas putida. The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The sample was then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using a Hewlett Packard 5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

对细菌进行糖基组成分析 来自恶臭假单胞菌的胞外多糖。 对样品进行了分析 通过制备三甲基甲硅烷基来制备单糖组合物 甲基糖苷衍生物的气相色谱分析 (GC) 和组合气相色谱/质谱 (GC-MS) 分析。 TMS 甲基糖苷由样品制备而成 在 3 M HCl 中进行甲醇分解，然后用吡啶进行 N-乙酰化 乙酸酐。 然后用 Tri-Sil 处理样品。 气相色谱 TMS 甲基糖苷分析是在 HP5890 GC 上使用 DB1 熔融石英毛细管柱。 GC-MS 分析使用 与 5970 MSD 连接的 Hewlett Packard 5890 GC。 单糖是 通过与标准品相比的保留时间来确定，以及 这些物质的碳水化合物特征是通过它们的质量来验证的 光谱。