CORE--MOUSE BREEDING, SCREENING AND MYCOPLASMA PULMONIS INFECTION

核心--小鼠选育、筛选和肺支原体感染

• 批准号: 6781172
• 负责人: NIGEL Patrick KILLEEN
• 金额: $ 14.27万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6781172
• 关键词: Mycoplasma; animal breeding; animal colony; biomedical facility; laboratory mouse

One unifying element of this program project is the use of Mycoplasma pulmonis-infected mice and rats as a model of chronic airway inflammation. All four projects will use this model to some extent in the proposed research. A small animal core will be established to meet this need. The ore has three main functions: (i) to standardize the infection procedures and handling of pathogen-free and infected mice and rats in the barrier facility; (ii) to maximize the efficiency and economy of housing and handling pathogen-free and infected animals; and (iii) to make pathogen-free and M. pulmonis-infect animals or tissues from these animals readily available to all of the investigators. The core staff will use tested stocks of M. pulmonis to ensure reproducible results and to minimize variability. The core staff will be responsible for husbandry and monitoring of pathogen-free and infected animals in the barrier facility, including the routine measurement of body weight, maintenance of sentinel animals, and periodic serological analyses. The core staff will also assist with some aspects of tissue harvesting, for example, bronchoalveolar lavage, flow cytometry, and tissue removal and fixation. Lastly, the core will assist in the breeding, colony management, and genetic analysis of various mutant animals stocks that will be used in the four projects. The core will build on an existing infrastructure that is already being used to generate M. pulmonis-infected mice and rats on a small scale. Centralizing and expanding the currently limited scope of the infection and maintenance process will avoid unnecessary replication of resources in the four projects. It will also ensure that common procedures are used by all of the groups and will, therefore, facilitate the exchange of information and foster collaborative interactions.

该程序项目的一个统一元素是使用支原体肺肺部感染的小鼠和大鼠作为慢性气道炎症的模型。在拟议的研究中，这四个项目将在某种程度上使用该模型。将建立一个小动物核心以满足这一需求。 该矿石具有三个主要功能：（i）标准化障碍物中无病原体和感染小鼠和大鼠的感染程序和处理； （ii）最大程度地提高住房的效率和经济性，并处理无病原体和感染动物的效率和经济； （iii）使所有研究人员易于使用这些动物的无病原体和肺部感染动物或组织。 核心人员将使用经肺杆菌的测试库存来确保可再现的结果并最大程度地减少变异性。 核心人员将负责烧烤和监测障碍设施中无病原体和感染动物的监测，包括常规测量体重，哨兵动物的维持以及定期的血清学分析。 核心人员还将有助于组织收集的某些方面，例如支气管肺泡灌洗，流式细胞仪以及切除和固定。最后，核心将有助于对四个项目中使用的各种突变动物库存的繁殖，菌落管理和遗传分析。 核心将建立在现有的基础设施上，该基础设施已经用于小型肺部感染小鼠和大鼠。集中和扩展当前有限的感染范围和维护过程将避免在四个项目中不必要的资源复制。 它还将确保所有小组都使用常见程序，并将促进信息的交换和促进协作互动。