The Action of Chinese Medicinal Herbs on Prostate Cancer

中药对前列腺癌的作用

• 批准号: 6834758
• 负责人: YAN-WEN DUAN
• 金额: $ 6.1万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-16 至 2007-03-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6834758
• 关键词: China; alternative medicine; antineoplastics; apoptosis; athymic mouse; combination chemotherapy; dosage; drug screening /evaluation; folk medicine; medicinal plants; neoplasm /cancer chemotherapy; neoplastic growth; nonhuman therapy evaluation; pharmacokinetics; postdoctoral investigator; prostate neoplasms; western blottings

DESCRIPTION (provided by applicant): Chinese herbal medicines have been widely used in the world. However, the efficacy and mechanisms of these herbal medicines are not well studied. It is imperative to investigate their efficacy using standardized design, and to elucidate the mechanisms of their action using modern technology. By employing an anti-tumor Chinese herbal formula, ZYD88, the current research proposal is designed to test the hypothesis that ZYD88 induces apoptosis in prostate cancer cells in a xenograft animal model with prostate LNCaP tumor. Cell apoptosis will be determined by assaying caspase-3 activation and CK18, the common early apoptotic events, and DNA fragmentation, a hallmark of cell apoptosis in tumor tissue sections. Furthermore, the release of cytochrome c from mitochondria to cytosol and the cellular levels of Bcl-2 family proteins (Bcl-2, BcI-XL, Bax and Bak), events in apoptotic pathway will be determined by Western blotting analysis. The data will be compared between ZYD88 treated and untreated samples. Dose-response and time-course analyses of ZYD88 action will be performed. These studies will facilitate our understanding of the efficacy and mechanisms of Chinese herbal medicine in cancer therapy, a goal of my career.

描述（申请人提供）：中草药已在世界范围内广泛使用。然而，这些草药的功效和机制尚未得到充分研究。必须使用标准化设计来研究它们的功效，并使用现代技术来阐明它们的作用机制。通过采用抗肿瘤中草药配方ZYD88，当前的研究计划旨在测试ZYD88在患有前列腺LNCaP肿瘤的异种移植动物模型中诱导前列腺癌细胞凋亡的假设。细胞凋亡将通过检测 caspase-3 激活和 CK18（常见的早期凋亡事件）和 DNA 碎片（肿瘤组织切片中细胞凋亡的标志）来确定。此外，细胞色素c从线粒体到细胞质的释放以及Bcl-2家族蛋白（Bcl-2、Bcl-XL、Bax和Bak）的细胞水平、凋亡途径中的事件将通过蛋白质印迹分析来确定。将比较 ZYD88 处理和未处理的样品之间的数据。将进行 ZYD88 作用的剂量反应和时程分析。这些研究将有助于我们了解中草药在癌症治疗中的功效和机制，这是我职业生涯的目标。