CHARACTERIZATION OF A NEW MOUSE MODEL FOR LUPUS

狼疮新小鼠模型的表征

• 批准号: 6605150
• 负责人: BING LIM
• 金额: $ 11.71万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-19 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6605150
• 关键词: SCID mouse; T cell receptor; apoptosis; autoantibody; autoimmune disorder; biological signal transduction; disease /disorder model; flow cytometry; gene expression; gene mutation; glomerulonephritis; linkage mapping; molecular cloning; molecular genetics; phenotype; polymerase chain reaction; systemic lupus erythematosus

DESCRIPTION (provided by applicant): The central goal of this proposal is to exploit the use of a new mutant murine strain to advance the understanding of autoimmune disorders. A new line of mice has been derived in which animals develop a severe generalized lymphadenopathy together with autoimmune glomerulonephritis and hyperimmunoglobulinemia. Significantly, the animals produce auto antibodies against double-stranded DNA and Sm antigen, both of which are specific markers for Systemic Lupus Erythematosus (SLE). Immune function studies showed a combination of severe lymphoid dysfunction and developmental defect not seen in other murine autoimmune disease models. The disease is passed with a Mendelian frequency consistent with a recessive mutation of an autosomal gene. Therefore, the disease arose from a spontaneous mutation of a gene which we have named lag (lymphoproliferative autoimmune glomerulonephropathy). Using chromosomal satellite markers to scan the murine genome, preliminary data indicate that a putative locus for the lag gene is the telomeric end of chromosome 2. This is not a region that has been linked before to autoimmune disease. The goal of this proposal is to exploit this remarkable new murine model to learn about autoimmune disease. In Specific Aim 1, we will map the location of the gene and identify the lag gene by combining positional cloning with a candidate gene approach. In Aim 2 we will characterize the disease process for the lag phenotype and identify the cells causing the disease. In Aim 3 we will examine in detail the effect of the lag mutation on T cell development. In Aim 4 we will investigate how the lag mutation affects T cell function. To support these studies, various TCRxlag transgenic animals will be generated to help the study of lymphocyte development, function and signaling. We anticipate that our proposal to study this murine model carefully will contribute a significant amount of new information for understanding the diverse genetic and molecular bases of autoimmune diseases. The identification of new genes and new pathways may uncover new targets for the development of drugs to suppress the immune system in a specific way, instead of globally. The discovery of new disease genes may also be very useful in the management, care and diagnosis of the large number of patients with autoimmune diseases.

描述（由申请人提供）：该提案的中心目标是利用新的突变小鼠品系来促进对自身免疫性疾病的理解。一个新的小鼠品系已被衍生出来，其中的动物会出现严重的全身性淋巴结病，并伴有自身免疫性肾小球肾炎和高免疫球蛋白血症。值得注意的是，这些动物产生针对双链 DNA 和 Sm 抗原的自身抗体，这两者都是系统性红斑狼疮 (SLE) 的特异性标志物。免疫功能研究显示，存在其他小鼠自身免疫性疾病模型中未见的严重淋巴功能障碍和发育缺陷的组合。该疾病以孟德尔频率传播，与常染色体基因的隐性突变一致。因此，这种疾病是由一种基因的自发突变引起的，我们将其命名为lag（淋巴增殖性自身免疫性肾小球肾病）。使用染色体卫星标记扫描小鼠基因组，初步数据表明，滞后基因的假定位点是 2 号染色体的端粒末端。此前，该区域并未与自身免疫性疾病相关。该提案的目标是利用这种非凡的新小鼠模型来了解自身免疫性疾病。在具体目标 1 中，我们将通过将位置克隆与候选基因方法相结合来绘制基因的位置图并识别滞后基因。在目标 2 中，我们将描述滞后表型的疾病过程并识别导致疾病的细胞。在目标 3 中，我们将详细研究滞后突变对 T 细胞发育的影响。在目标 4 中，我们将研究滞后突变如何影响 T 细胞功能。为了支持这些研究，将产生各种 TCRxlag 转基因动物来帮助研究淋巴细胞的发育、功能和信号传导。我们预计，仔细研究这种小鼠模型的建议将为了解自身免疫性疾病的不同遗传和分子基础提供大量新信息。新基因和新途径的识别可能会发现药物开发的新靶点，以特定方式而不是全局方式抑制免疫系统。新疾病基因的发现也可能对大量自身免疫性疾病患者的管理、护理和诊断非常有用。