Novel DNA-Based Detector Library in an Artificial Nose

人工鼻中基于 DNA 的新型探测器库

基本信息

批准号：
7056783
负责人：
LLOYD B WILLIAMS
金额：
$ 3.77万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-05-01 至 2008-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7056783
关键词：
DNA bioengineering /biomedical engineering biohazard detection chemical stability combinatorial chemistry diagnostic tests disease /disorder prevention /control environmental protection food quality /standard genetic library high throughput technology humidity intermolecular interaction ionophores microarray technology molecular polarity monitoring device nucleic acid sequence nucleic acid structure odors predoctoral investigator public health safety equipment solvents technology /technique development temperature

DNA bioengineering /biomedical engineering biohazard detection chemical stability combinatorial chemistry diagnostic tests disease /disorder prevention /control environmental protection food quality /standard genetic library high throughput technology humidity intermolecular interaction ionophores microarray technology molecular polarity monitoring device nucleic acid sequence nucleic acid structure odors predoctoral investigator public health safety equipment solvents technology /technique development temperature

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项目摘要

DESCRIPTION (provided by applicant): Many potential applications for tunable odor detectors exist in medical diagnosis, toxic and explosive chemical detection, and food processing. Preliminary work for this application has developed the first known single-stranded DNA-Cy3 odor sensors for an artificial olfactory system. These DNA-Cy3 conjugates will provide a large array of novel odorant sensors dependent on DNA sequence and other conditions. A high throughput odor response screening process based on standard DNA library and microarray technologies is proposed here. This screening process will be used to identify sequences that have applications in a number of real-world health problems. Emission spectral analysis and DNA base swapping experiments will also be used to study mechanisms of DNA-Cy3 sensor odor response. Better understanding of the mechanisms involved in DNA-Cy3 odorant sensing will be valuable in developing an optimized detector system. The large array of sensors available in a DNA-based detector system will provide a powerful tool to find odorant sensors that will address health issues like lung cancer diagnosis, toxin and landmine detection, and spoilage in the food processing industry.

描述（由申请人提供）：可调气味探测器的许多潜在应用中存在于医学诊断，有毒和爆炸性化学检测以及食物加工中。该应用程序的初步工作已经开发了第一个已知的单链DNA-CY3气味传感器，用于人工嗅觉系统。这些DNA-CY3偶联物将提供一系列依赖于DNA序列和其他条件的新型气味传感器。这里提出了基于标准DNA库和微阵列技术的高吞吐量响应筛选过程。此筛选过程将用于识别在许多现实世界中具有应用程序中具有应用程序的序列。发射光谱分析和DNA碱基交换实验也将用于研究DNA-CY3传感器气味反应的机制。更好地理解DNA-CY3气味传感所涉及的机制对于开发优化的检测器系统将是有价值的。基于DNA的探测器系统中可用的大量传感器将提供一个有力的工具，可以找到可以解决诸如肺癌诊断，毒素和地雷检测的健康问题的气味传感器，以及食品加工行业的变质。