CORE--CELL ISOLATION AND CULTURE

核心——细胞分离与培养

• 批准号: 6808274
• 负责人: GEORGE COOPER
• 金额: $ 9.95万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6808274
• 关键词: biomedical facility; heart cell; heart enlargement; intracardiac pressure; myocardium; pathologic process; tissue /cell culture; tissue /cell preparation

The majority of the program projects will utilize experimental preparations that require isolation and characterization of adult and neonatal cardiac muscle cells, or cardiocytes. This core will isolate cardiocytes from: 1) the adult cat, 2) the adult mouse, 3) the neonatal rat, and 4) the neonatal mouse. Adult cardiocytes will be isolated from both normal hearts and surgical models of pressure overload hypertrophy. These demands will best be met by a Core Facility where supply purchases, reagent preparation, and routine procedures can be performed with an economy based on scale, with quality control monitored from a centralized, experienced authority, and with time saved by the provision of labor-intensive services in a centralized facility.

大多数计划项目将利用需要分离和表征成人和新生儿心肌细胞或心肌细胞的实验制剂。该核心将从以下来源分离心肌细胞：1) 成年猫，2) 成年小鼠，3) 新生大鼠，4) 新生小鼠。成人心肌细胞将从正常心脏和压力超负荷肥大的手术模型中分离出来。这些需求将通过核心设施得到最好的满足，在该设施中，物资采购、试剂制备和常规程序可以以基于规模的经济方式进行，质量控制由集中的、经验丰富的权威机构监控，并通过提供劳动力节省时间-集中设施中的密集服务。