HIGHLY MULTIPLEXED GENOTYPING PLATFORM

高度多重基因分型平台

• 批准号: 6935106
• 负责人: STEVEN C MILLER
• 金额: $ 15.21万
• 依托单位: BLUESHIFT BIOTECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-19 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935106
• 关键词: biohazard detection; fluorescent dye /probe; genetic screening; genotype; high throughput technology; ionophores; lasers; microorganism genetics; nucleic acid sequence; technology /technique development

DESCRIPTION (provided by applicant): This SBIR Phase I project proposes to develop a novel technology platform for very high throughput multiplexing important for genotyping and pathogen detection. A unique barcoding scheme for bead-based assays with the potential of individually labeling millions of samples is combined with a high speed laser scanning instrument developed by Blueshift Biotechnologies, Inc. (BBI). The proposed scanning system will combine two lasers: one for interrogating fluorophore labeled oligonucleotides, and the other for measuring bead encoding. The robust and cost-effective design will enable fast readout of very highly multiplexed samples and provide a workable platform for highly multiplexed genotyping applications. The system also will employ a novel anisotropy measurement scheme to provide superior discrimination of incorporated from free fluorophore. This powerful technique enables high throughput genotyping, such as the typing of microbial and viral pathogens by sequence detection in homogeneous assay formats. The combination of fast sequence signature detection in homogeneous assays will provide a powerful commercial platform for pharmacogenomics, biodefense, diagnostics, and other medically important applications. The optical encoding system is based on rare earth upconverting phosphor (RUP) materials. The unique optical properties of RUPs enable high throughput particle multiplexing, identifying >105 times more samples than the best current optical barcoding scheme. The use of multiplexing based on the ratios of more than one RUP within a single particle has been developed by Parallel Synthesis Technologies, Inc. (PSTI). They have demonstrated that it is possible to resolve >300 individual ratios from a two-color RUP system. It is estimated that about 3 x 10/7 and 6 x 10/9 uniquely labeled samples are possible with a four- or five-color RUP system, respectively.

描述（由申请人提供）：这个SBIR I期项目提议开发一个新型的技术平台，以使高通量多路复用对于基因分型和病原体检测很重要。用于基于珠的测定的独特条形码方案，具有单独标记数百万个样品的潜力与由Blueshift Biotechnologies，Inc。（BBI）开发的高速激光扫描仪器结合使用。提出的扫描系统将结合两个激光器：一种用于询问标有寡核苷酸的荧光团，另一个用于测量珠编码。强大且具有成本效益的设计将使非常高度多重的样本快速读数，并为高度多重的基因分型应用提供了可行的平台。该系统还将采用一种新颖的各向异性测量方案来提供对自由荧光团的合并的优越歧视。这种强大的技术可以实现高通量基因分型，例如通过均匀测定格式通过序列检测进行微生物和病毒病原体的键入。在均质测定中快速序列签名检测的结合将为药物基因组学，生物探索，诊断和其他医学上重要的应用提供强大的商业平台。 光学编码系统基于稀土上转换磷光器（RUP）材料。 RUP的独特光学特性使高吞吐量粒子多路复用，比最佳当前光学条形码方案确定了> 105倍的样品。平行合成技术公司（PSTI）开发了基于单个粒子中多个RUP比率的多路复用。他们已经证明，可以从两色RUP系统中解析> 300个个人比率。据估计，分别有四个或五色的RUP系统，可能可以使用大约3 x 10/7和6 x 10/9唯一标记的样品。