Dental Anxiety, Pain & Melanocortin-1 Receptor Mutations

牙科焦虑、疼痛

基本信息

批准号：
6949180
负责人：
EDWIN B LIEM
金额：
$ 11.03万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-15 至 2007-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6949180
关键词：
adrenocorticotropic hormone anesthesia behavioral /social science research tag clinical research dental anxiety drug administration rate /duration gene mutation genetic screening genetic susceptibility hair health behavior health care service utilization hormone receptor human subject oral facial pain oral health pain threshold questionnaires

项目摘要

DESCRIPTION (provided by applicant): This exploratory biobehavioral pain research will study differences anxiety pain in dental and in natural red-haired men and women compared with dark-haired men and women. Red hair results from mutations of the melanoncortin-1 receptor gene (MC1R). Our specific aims are to 1) determine the association between perceived dental pain, dental anxiety, and dental utilization with MC1R gene status and hair color; 2) determine experimental pain thresholds and dose-dependent local anesthetic sensitivity as a function of hair color and MC1R gene variation in volunteers; and 3) determine local anesthetic requirement for restorative dental treatment as a function of hair color and MC1R gene variation. This proposal is collaboration between the School of Dentistry, the Department of Anesthesiology, the OUTCOMES RESEARCH Institute, the Genetics Core Laboratory, and the Department of Psychology -- all at the University of Louisville. All participating male and female subjects will be genotyped for MC1R alleles, and new technology will be used that provides consistent and objective assessment of dental pain. The study will be accomplished in three phases: 1) In the Observational Phase, 74 redheads and 74 dark-haired participants (aged 18 to 40) will each complete validated survey instruments designed to measure psychological symptomatology, global and dental-specific anxiety, constructs associated with dental pain and avoidance (e.g., anxiety and pain sensitivity), and previous dental history including dental care utilization. 2) In the Experimental Phase, we will recruit 60 redheaded and 60 dark-haired volunteers from those completing the Observational phase. Pain, perceived as a result of electrical current and thermal stimuli on unrestored lateral incisor teeth, will be measured after intra-oral injection of various lidocaine concentrations. The concentrations, which will be used in a randomized and double-blinded sequence, will be 0, 0.5, 1.0, and 1.5%. And 3) in the Clinical Phase, we will recruit 60 redheaded and 60 dark-haired volunteers requiring non-surgical dental treatment from those completing the Observational phase. We will use the Dixon "up-and-down" method to determine local anesthetic requirement in each group. The proposed research will contribute to the knowledge of the role of gender and genetics in peripheral nerve function, pain perception, and behavior. The research will also explore the feasibility of an innovative dental pain assessment approach that may provide a basis for future research projects and could guide the development of biobehavioral interventions to manage dental anxiety and prevent pain.

描述（由申请人提供）：这项探索性生物行为疼痛研究将研究与黑发男性和女性相比，牙科和自然红发男性和女性的焦虑疼痛差异。红头发是黑皮质素-1 受体基因 (MC1R) 突变的结果。我们的具体目标是 1) 确定感知的牙痛、牙科焦虑和牙科使用与 MC1R 基因状态和头发颜色之间的关联； 2) 确定实验疼痛阈值和剂量依赖性局部麻醉敏感性作为志愿者毛发颜色和 MC1R 基因变异的函数； 3) 根据头发颜色和 MC1R 基因变异确定修复性牙科治疗的局部麻醉需求。该提案是路易斯维尔大学牙科学院、麻醉学系、结果研究所、遗传学核心实验室和心理学系之间的合作。所有参与的男性和女性受试者都将进行 MC1R 等位基因的基因分型，并将使用新技术来提供一致且客观的牙痛评估。该研究将分三个阶段完成：1) 在观察阶段，74 名红发参与者和 74 名黑发参与者（年龄 18 至 40 岁）将各自完成经过验证的调查工具，旨在测量心理症状、整体和牙科特定焦虑、构建与牙科疼痛和回避（例如焦虑和疼痛敏感性）以及既往牙科病史（包括牙科护理利用）相关。 2）在实验阶段，我们将从完成观察阶段的志愿者中招募60名红发志愿者和60名黑发志愿者。在口腔内注射不同浓度的利多卡因后，将测量未修复的侧切牙上因电流和热刺激而产生的疼痛。将用于随机双盲序列的浓度为 0、0.5、1.0 和 1.5%。 3) 在临床阶段，我们将从完成观察阶段的志愿者中招募 60 名红发志愿者和 60 名黑发志愿者，需要非手术牙科治疗。我们将使用 Dixon“上下”方法来确定每组的局部麻醉需求。拟议的研究将有助于了解性别和遗传学在周围神经功能、疼痛感知和行为中的作用。该研究还将探索创新牙痛评估方法的可行性，该方法可能为未来的研究项目提供基础，并可以指导生物行为干预措施的开发，以管理牙科焦虑和预防疼痛。