The Regulation of gene expression in Chlamydia

衣原体基因表达的调控

• 批准号: 6899867
• 负责人: Ming Tan
• 金额: $ 36.78万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6899867
• 关键词: Chlamydiaceae; DNA directed RNA polymerase; DNA footprinting; affinity chromatography; bacterial genetics; bioinformatics; developmental genetics; functional /structural genomics; gel mobility shift assay; gene mutation; genetic regulation; genetic regulatory element; genetic transcription; heat shock proteins; microarray technology; protein purification; transcription factor

DESCRIPTION (provided by applicant): Chlamydia is the leading cause of sexually transmitted disease in the developed world, and preventable blindness in the developing world. Our long-term goal is to define the molecular mechanisms that regulate chlamydial gene expression so that we may intervene during the organism's intracellular developmental cycle. Our central hypothesis is that chlamydial gene expression is coordinately regulated at the transcriptional level by master regulatory molecules, such as activators and repressors, and by alternative forms of RNA polymerase. We propose three aims: 1) Investigate the function of a cis-acting DNA element that is important for Chlamydia-specific promoter activity. We will use biochemical and physical approaches to determine if a novel DNA element called the Spacer A/T region exerts its positive effect on transcription by binding an activator. 2) Define the role of HrcA, a transcription factor that regulates the expression of heat shock genes. We will determine how heat shock gene expression is regulated by the physical state of the HrcA repressor, higher temperature, DNA topology, and by the heat shock protein, GroEL. 3) Define the role of sigma28 RNA polymerase, an alternative RNA polymerase. We will use functional, bioinformatics and DNA microarray approaches to identify sigma28 promoters and sigma28-regulated genes.

描述（由申请人提供）：衣原体是发达国家性传播疾病的主要原因，并且是发展中国家的可预防失明。我们的长期目标是定义调节衣原体基因表达的分子机制，以便我们可以在生物体的细胞内发育周期中进行干预。我们的中心假设是，衣原体基因表达是由主调节分子（例如激活剂和抑制剂）以及RNA聚合酶的替代形式在转录水平上协调调节的。我们提出了三个目标： 1）研究对衣原体特异性启动子活性很重要的顺式作用DNA元件的功能。 我们将使用生化和物​​理方法来确定一种新型的DNA元素是否通过结合激活剂来对转录产生对转录的积极作用。 2）定义HRCA的作用，HRCA是调节热休克基因表达的转录因子的作用。我们将确定热休克基因表达如何受HRCA阻遏物，较高温度，DNA拓扑的物理状态以及热休克蛋白Groel调节。 3）定义Sigma28 RNA聚合酶（一种替代RNA聚合酶）的作用。我们将使用功能性，生物信息学和DNA微阵列方法来鉴定Sigma28启动子和SigMA28调节的基因。