Proteins involved in plant perception of pathogen

参与植物感知病原体的蛋白质

• 批准号: 6883537
• 负责人: AMY K DEHART
• 金额: $ 4.17万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2006-01-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6883537
• 关键词: SDS polyacrylamide gel electrophoresis; cell morphology; chemical binding; green fluorescent proteins; host organism interaction; immunity; immunogenetics; immunoprecipitation; mass spectrometry; molecular shape; plant virus; plants; polymerase chain reaction; postdoctoral investigator; protein protein interaction; protein structure function; tobacco mosaic virus; virus diseases; virus infection mechanism; yeast two hybrid system

DESCRIPTION (provided by applicant): Understanding the recognition and signaling events that allow a plant to successfully combat infection is a high priority in plant research, and can contribute to reducing the vulnerability of plants to disease and resulting economic loss, and use of pesticides. This proposal describes a biochemical approach to elucidate the mechanism by which one resistance protein (N) is able to recognize and combat infection of the tobacco mosaic virus (TMV). Increasing evidence for several R genes has indicated that they often function not by recognizing the pathogen directly, but by recognizing specific cellular modifications elicited by the pathogen. The mechanism by which N is able to recognize the presence of TMV is not clear. This proposal aims to characterize this mechanism by identifying 1) N-interacting proteins and 2) TMV-interacting factors in the cell. Interacting proteins are likely to include downstream signaling components of disease resistance pathways as well as cellular targets of TMV action in the cell. Knowledge of this mechanism will not only benefit defense against TMV, but also potentially many other diseases, since both resistance genes themselves and the signaling pathways they induce are highly conserved across plant species.

描述（由申请人提供）：了解植物成功抵抗感染的识别和信号事件是植物研究的重中之重，并且有助于减少植物对疾病的脆弱性以及由此造成的经济损失和农药的使用。该提案描述了一种生化方法来阐明一种抗性蛋白 (N) 能够识别和对抗烟草花叶病毒 (TMV) 感染的机制。越来越多的证据表明，一些 R 基因通常不是通过直接识别病原体来发挥作用，而是通过识别病原体引起的特定细胞修饰来发挥作用。 N 能够识别 TMV 存在的机制尚不清楚。该提案旨在通过识别细胞中的 1) N 相互作用蛋白和 2) TMV 相互作用因子来表征该机制。相互作用的蛋白质可能包括抗病途径的下游信号成分以及 TMV 在细胞中作用的细胞靶标。了解这种机制不仅有利于防御TMV，而且可能有利于许多其他疾病，因为抗性基因本身及其诱导的信号通路在植物物种中高度保守。