Molecular-genetic analysis of the Drosophila CNS midline

果蝇中枢神经系统中线的分子遗传学分析

• 批准号: 6993909
• 负责人: SCOTT R WHEELER
• 金额: $ 4.4万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6993909
• 关键词: Drosophilidae; apoptosis; arthropod genetics; cell cell interaction; cell differentiation; cell growth regulation; cell migration; central nervous system; developmental genetics; gene expression; genetic mapping; neurogenesis; neurons; postdoctoral investigator; protein structure function; tissue /cell culture; transcription factor

DESCRIPTION (provided by applicant): In this proposal I will use the CMS midline cells to study the genetic basis of neurogenesis and the generation of neural cell type diversity. The development of the Drosophila CMS midline cells illustrates a large number of key developmental processes including cell-cycle control, cell-cell signaling, cell migration, and apoptosis. Together with the genetic and genomic resources available to the Drosophila community, the ease of identifying genes expressed in these cells, and their location along the ventral midline the Drosophila CMS midline cells have the potential to be an excellent developmental and genomic model system. This proposal is designed to generate a detailed map of midline cell gene expression, fate, and cellular dynamics such that each midline cell and its progenitors can be identified. I will use this information to isolate and characterize the functional role of transcription factors that promote cell fate and differentiation in midline neurons. Through the combination of descriptive and functional-genetic studies I will begin to dissect the molecular and genetic pathways that control the development and differentiation midline neurons.

描述（由申请人提供）：在此提案中，我将使用CMS中线细胞研究神经发生的遗传基础和神经细胞类型多样性的产生。果蝇CMS中线细胞的开发说明了大量关键的发育过程，包括细胞周期控制，细胞 - 细胞信号传导，细胞迁移和凋亡。连同果蝇群落可用的遗传和基因组资源，识别这些细胞中表达的基因的易度性以及它们沿着腹部中线的位置果蝇中线细胞具有出色的发展和基因组模型系统。该建议旨在生成中线细胞基因表达，命运和细胞动力学的详细图，以便可以鉴定出每个中线细胞及其祖细胞。我将使用此信息来隔离并表征转录因子的功能作用，这些转录因子促进了中线神经元中细胞命运和分化。通过描述性和功能遗传学研究的结合，我将开始解剖控制发展和分化中线神经元的分子和遗传途径。