Interaction Between HSV Glycoproteins and Tequment

HSV 糖蛋白和 Tequment 之间的相互作用

• 批准号: 6891428
• 负责人: MARIAM L NIX
• 金额: $ 3.87万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-28 至 2006-03-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6891428
• 关键词: biochemistry; genetic mapping; glycoproteins; herpes simplex virus 1; membrane proteins; peptides; protein binding; protein protein interaction; recombinant DNA; virion; virus envelope; virus protein

DESCRIPTION (provided by applicant): The Herpes simplex virus (HSV) particle consists of a DNA-containing capsid surrounded by a lipid envelope which bears a diverse array of membrane proteins. Lining the inner surface of the envelope is a complex and poorly characterized layer of polypeptides termed tegument. The HSV envelope is acquired by budding of capsids through host cell organellar membranes, and envelope formation is essential for the subsequent transmission of viral disease. However, little is known of the molecular details of envelope assembly. A key step in envelope formation is likely to be the binding of tegument polypeptides to the cytoplasmic tails of viral envelope glycoproteins. This recruitment of tegument onto the membrane may then provide a binding site for the viral capsid. We have confirmed this hypothesis by developing assay systems which reconstitute the interaction of glycoprotein tails, tegument proteins and HSV capsids in vitro. With these assay systems available we now propose to use biochemical and recombinant DNA techniques to investigate the molecular events which take place during HSV particle assembly.

描述（由申请人提供）：单纯疱疹病毒（HSV）粒子 由含有DNA的衣壳包围的含有DNA的衣壳组成 各种各样的膜蛋白。衬里信封的内表面 是一种称为Tegument的多肽层的复杂且特征性的层。 HSV信封是通过宿主细胞细胞器的带壳的萌芽而获得的 膜和信封形成对于随后的传播至关重要 病毒疾病。但是，对信封的分子细节知之甚少 集会。 信封形成的关键步骤可能是Tegument的结合 病毒包膜糖蛋白的细胞质尾部的多肽。这 然后，将Tegument招募到膜上可能会提供一个约束位点 病毒式衣壳。我们通过开发测定系统证实了这一假设 重建了糖蛋白尾巴，Tegument蛋白和 体外HSV衣壳。使用这些分析系统，我们现在建议使用 生化和重组DNA技术研究分子事件 在HSV粒子组件中发生的。