Virus Dispersal in Genetically Diverse Host Populations

病毒在遗传多样性宿主群体中的传播

• 批准号: 6942242
• 负责人: GEROD S HALL
• 金额: $ 3.66万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6942242
• 关键词: Gramineae; Reoviridae; genetic strain; insect virus; mathematical model; plant population genetics; plant virus; transfection /expression vector; virus genetics; virus infection mechanism

DESCRIPTION (provided by applicant): This study will characterize the dispersal of an insect-borne plant virus in genetically diverse host populations and, in so doing, explore how vector-host relationships influence the propagation and maintenance of a disease epidemic. I will carry out a series of field and greenhouse experiments to explore the role of aphid infection prevalence, aphid movement and feeding behavior in determining the spread of barley yellow dwarf virus (BYDV) in genetically diverse stands of oats. The oat varieties used will either be susceptible, tolerant or resistant to infection by BYDV. Mixed stands of three different varieties of oats will be planted in the field and colonized by natural aphid populations. It will be necessary to measure aphid infection prevalence and disease incidence in the oat stands throughout the course of the epidemic. It will also be necessary to determine the spatial distribution of aphids within diseased and healthy patches within the stand. In order to determine if the acceptability of the variety changes as with its infection status, greenhouse experiments will compare feeding behavior of two aphid species on infected and uninfected plants of each variety listed above. This knowledge will grant new insight into the population dynamics of vector-borne pathogens and may in turn assist public health officials in developing more effective disease management strategies.

描述（由申请人提供）： 这项研究将表征昆虫传播的植物病毒在遗传多样的宿主种群中的扩散，并在这样做时探索媒介 - 寄养关系如何影响疾病流行病的传播和维持。我将进行一系列田间和温室实验，以探讨蚜虫感染的患病率，蚜虫运动和喂养行为在确定大麦黄色矮人病毒（BYDV）在燕麦的遗传不同林分中的传播中的作用。所使用的燕麦品种要么易感，耐受性或耐药性。混合林体将种植三种不同品种的燕麦，并在天然蚜虫种群中殖民。在整个流行病的过程中，有必要测量燕麦的蚜虫感染的患病率和疾病发病率。还必须确定在看台内患病和健康斑块中蚜虫的空间分布。为了确定多样性的可接受性是否随着其感染状态而变化，温室实验将比较上面列出的每种品种的感染和未感染植物的两种蚜虫物种的喂养行为。这些知识将为媒介传播病原体的人群动态提供新的见解，反过来又可能有助于公共卫生官员制定更有效的疾病管理策略。