Protein Cages for Targeted Drug Delivery & Imaging

用于靶向药物输送的蛋白质笼

• 批准号: 6937011
• 负责人: Michelle Flenniken
• 金额: $ 2.73万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6937011
• 关键词: Bromovirus; SDS polyacrylamide gel electrophoresis; bioengineering /biomedical engineering; biomaterial development /preparation; biotechnology; capsid; clathrate; computer program /software; doxorubicin; drug delivery systems; heat shock proteins; iron oxide; mass spectrometry; nanotechnology; predoctoral investigator; reagent /indicator; technology /technique development; tissue /cell culture; transfection /expression vector; transmission electron microscopy; transport proteins; virus protein

DESCRIPTION (provided by applicant): In the next twenty years, the medical field will be transformed by the use of nanotechnologies to diagnose, image, treat, and prevent diseases and disorders. The generation of these nanotechnologies requires basic research at the interfaces of biology, chemistry, physics, and engineering. The long-term objective of this research is to develop nanometer scale protein cage architectures for targeted delivery of therapeutic compounds and imaging agents. The overall goal of this proposal is to employ genetic and chemical modifications to impart biomedical functions to selected protein cages. Two protein cage systems will be utilized, the 28 nm diameter cowpea chlorotic mottle viral capsid devoid its nucleic acid (CCMV) and the 12 nm diameter small heat shock protein (MjHsp). These protein cages will be used to encapsulate therapeutics (doxorubicin), imaging agents (iron oxides) and present tumor-targeting peptides on their exterior surface. These constructs will be tested in cell culture assays for their ability to direct cell targeted drug delivery and imaging.

描述（由申请人提供）： 在接下来的二十年中，医学领域将通过使用纳米技术来诊断、成像、治疗和预防疾病和失调而发生改变。这些纳米技术的产生需要在生物学、化学、物理学和工程学的交叉领域进行基础研究。这项研究的长期目标是开发纳米级蛋白笼结构，用于靶向递送治疗化合物和显像剂。该提案的总体目标是利用遗传和化学修饰来赋予选定的蛋白质笼生物医学功能。将使用两种蛋白笼系统：直径 28 nm 的无核酸豇豆褪绿斑驳病毒衣壳 (CCMV) 和直径 12 nm 的小热休克蛋白 (MjHsp)。这些蛋白笼将用于封装治疗药物（阿霉素）、显像剂（氧化铁），并在其外表面上呈现肿瘤靶向肽。这些构建体将在细胞培养测定中测试其指导细胞靶向药物递送和成像的能力。