Identifying Novel Protein Lysine Methyltransferases

鉴定新型蛋白质赖氨酸甲基转移酶

基本信息

批准号：
6992565
负责人：
Tanya Rocio Porras-Yakushi
金额：
$ 2.96万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-01 至 2007-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6992565
关键词：
SDS polyacrylamide gel electrophoresis Saccharomyces cerevisiae autoradiography enzyme activity enzyme substrate fungal genetics gene deletion mutation genetic translation lysine matrix assisted laser desorption ionization methylation methyltransferase mutant predoctoral investigator protein structure function ribosomal RNA ribosomal proteins thin layer chromatography

项目摘要

DESCRIPTION (provided by applicant): The role that lysine methylation of ribosomal and ribosomal-associated proteins plays in regulating the process of translation is poorly understood compared to its role in regulating other processes such as transcription. The purpose of this proposal is to identify novel protein lysine methyltransferases in Saccharomyces cerevisiae that may be involved in regulating translation. This will be accomplished by analyzing knockout strains in which putative SET domain-containing genes have been deleted. SET domain-containing methyltransferases are lysine methyltransferases that have been shown to post-translationally modify proteins such as cytochrome c, Rubisco, and histones. During a search of the Protein Family Database, seven genes encoding putative lysine methyltransferases were found, one of which I have already determined to encode a ribosomal protein lysine methyltransferase, which I now refer to as RKM1. One aim of this proposal will be to determine whether the remaining genes that have the closest homology to RKM1 also encode ribosomal protein lysine methyltransferases. Once the substrates are identified I plan to determine the role the methyltransferase plays in the regulation of its substrate and subsequently translation.

描述（由申请人提供）：与核糖体和核糖体相关蛋白的赖氨酸甲基化在调节其他过程（例如转录）中的作用相比，人们对核糖体和核糖体相关蛋白的赖氨酸甲基化在调节翻译过程中所起的作用知之甚少。该提案的目的是鉴定酿酒酵母中可能参与调节翻译的新型蛋白质赖氨酸甲基转移酶。这将通过分析敲除菌株来实现，其中推定的包含 SET 结构域的基因已被删除。包含 SET 结构域的甲基转移酶是赖氨酸甲基转移酶，已被证明可以对细胞色素 c、Rubisco 和组蛋白等蛋白质进行翻译后修饰。在蛋白质家族数据库的搜索过程中，发现了七个编码假定的赖氨酸甲基转移酶的基因，其中一个我已经确定编码核糖体蛋白赖氨酸甲基转移酶，我现在将其称为 RKM1。该提案的一个目的是确定与 RKM1 具有最接近同源性的其余基因是否也编码核糖体蛋白赖氨酸甲基转移酶。一旦确定了底物，我计划确定甲基转移酶在其底物调节和随后的翻译中所起的作用。