Confocal Core Facility for Vision Researchers

视觉研究人员的共焦核心设施

• 批准号: 6653653
• 负责人: A. Sue Menko
• 金额: $ 57.51万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653653
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; biomedical facility; confocal scanning microscopy; fluorescence microscopy; health science research; vision

DESCRIPTION (provided by applicant): This R24 Grant is submitted to establish a state-of-the-art Confocal Laser Scanning Microscope Core Facility for vision researchers at Thomas Jefferson University (TJU). It will provide a well-established group of NEI-funded vision researchers at TJU the means to perform cutting edge fluorescence microscopy analysis of both fixed and live cells and tissues, greatly enhancing their vision research programs. This facility will also serve collaborators and colleagues of the NEI-funded investigators who have an interest in vision biology. It is hoped that the advanced technology offered by this facility will attract additional investigators at TJU to apply their knowledge to questions in vision research, either independently or in collaboration with the core group of investigators. The confocal microscope we propose to purchase, the Zeiss LSM 510 META, well addresses the needs of the vision investigators at TJU to examine the complex and dynamic relationships between proteins at the cellular, subcellular and molecular level. It offers the unique capability of emission fingerprinting which can separate up to 8 different fluorescent markers despite overlap of their emission spectra, thereby permitting analysis of the molecular relationships of distinct cellular components. This microscope makes it simple to perform dynamic FRAP, FLIP and FRET analyses. It can resolve rapid cellular processes and allows for quantitative analysis of image series. It is expected that this confocal facility not only will enhance the current research goals of this group of vision researchers but also provide new avenues for their research projects not before possible. Access to this confocal microscope should allow this dynamic group of vision researchers, who have already provided many important contributions to their specific fields, to both enhance and expand their research directions and to increase their productivity, without technology being a limitation. It is our long term goal, as we reach the required nucleus of NEI-funded investigators, to expand this facility into a larger Core Facility for vision researchers at TJU and Wills Eye Hospital. We envision this core as providing not only additional technologies that will enhance the research activity of these investigators, but also to provide a central site that will foster intellectual interactions between the investigators and lead to even greater cooperation and collaboration within the TJU vision community.

描述（由申请人提供）：此 R24 拨款旨在为托马斯杰斐逊大学 (TJU) 的视觉研究人员建立最先进的共焦激光扫描显微镜核心设施。它将为 TJU 的 NEI 资助的视觉研究人员团队提供对固定和活细胞和组织进行尖端荧光显微镜分析的手段，从而极大地增强他们的视觉研究项目。该设施还将为 NEI 资助的对视觉生物学感兴趣的研究人员的合作者和同事提供服务。希望该设施提供的先进技术能够吸引天津大学的更多研究人员独立或与核心研究人员小组合作，将他们的知识应用于视觉研究问题。我们建议购买的共焦显微镜 Zeiss LSM 510 META 很好地满足了 TJU 视觉研究人员在细胞、亚细胞和分子水平上检查蛋白质之间复杂和动态关系的需求。它提供独特的发射指纹识别功能，尽管发射光谱重叠，但仍可分离多达 8 种不同的荧光标记，从而允许分析不同细胞成分的分子关系。该显微镜可以轻松执行动态 FRAP、FLIP 和 FRET 分析。它可以解析快速的细胞过程，并允许对图像系列进行定量分析。预计该共焦设备不仅将增强这组视觉研究人员当前的研究目标，而且还将为他们的研究项目提供以前不可能的新途径。使用这种共焦显微镜应该可以让这群充满活力的视觉研究人员（他们已经为其特定领域做出了许多重要贡献）增强和扩展他们的研究方向并提高他们的生产力，而不受技术的限制。我们的长期目标是，当我们达到所需的 NEI 资助的研究人员核心时，将该设施扩展为 TJU 和 Wills 眼科医院视觉研究人员的更大核心设施。我们设想这个核心不仅提供额外的技术来增强这些研究人员的研究活动，而且还提供一个中心站点，促进研究人员之间的智力互动，并在 TJU 视觉社区内带来更大的合作与协作。