CORE--PREPARATIVE FACILITIES

核心——准备设施

• 批准号: 7010372
• 负责人: Zijian Xie
• 金额: $ 35.74万
• 依托单位: UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2008-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7010372
• 关键词: biomedical facility; cardiac myocytes; heart cell; hydrogen potassium exchanging ATPase; protein purification; sodium potassium exchanging ATPase; tissue /cell culture; tissue /cell preparation

DESCRIPTION (provided by applicant) The purpose of this core remains the centralization of repetitive preparations of several items that are "starting materials" and are used by one or more of the projects. We have found this approach to be efficient and convenient for all concerned. It allows the core technicians to master a small number of difficult tasks; when one is temporarily absent or needs to be replaced the work does not stop; and there is a continuous supply of high quality material available to the users. The common core facility, equipment, and routine supplies, administered and supervised by a research scientist with appropriate expertise, is also quite economical. The preparations to be made by the core are: 1. Primary cultures of neonatal rat cardiac myocytes, and adult rat cardiac myocytes either in suspension or as plated cultures, to be used by Projects 1, 11, and some of the experiments of Project 12 that will be done in Toledo. 2. Preparations of crude Sf-21 insect cell membranes containing the recombinant H+/K+-ATPase to be used by Project 10. 3. Preparation of purified recombinant Src expressed in Sf-9 cells, to be used primarily by Project 10. 4. Preparations of cardiac sarcolemmal membranes from beef and rat heart ventricles, to be used primarily by Project 1. 5. Preparation of purified Na+/K+-ATPase from pig kidney, to be used primarily by Project 11, and to smaller extents by Projects 1 and 10.

描述（由申请人提供） 该核心的目的仍然是几个项目重复准备的集中化 是“起始材料”，并由一个或多个项目使用。我们发现了这个 对于所有有关的人来说，有效且方便的方法。它允许核心技术人员 掌握少量艰巨的任务；当一个人暂时缺席或需要 替换工作不会停止；而且有不断的高质量材料 可用于用户。公共核心设施，设备和常规用品， 由具有适当专业知识的研究科学家管理和监督，也很 经济。核心进行的准备工作是：1。新生大鼠的主要培养物 心肌细胞和成年大鼠心肌细胞悬浮或镀培养物， 项目1、11和项目12的一些实验将在 托莱多。 2。原油SF-21昆虫细胞膜的制剂含有重组 h+/k+-ATPase将由项目10。3。纯化的重组SRC的制备 SF-9细胞，主要由项目10。4。心脏肌肉制剂的制剂使用 牛肉和大鼠心室的膜，主要由项目1。5。 从猪肾脏纯化的Na+/K+-ATPase，主要由项目11使用，而较小 项目1和10。