Mechanisms of Nucleic acid Uptake by Renal Epithelium

肾上皮摄取核酸的机制

• 批准号: 6844846
• 负责人: PAUL Evan KLOTMAN
• 金额: $ 32.84万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-20 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6844846
• 关键词: DNA; biological transport; epithelium; gene targeting; genetically modified animals; high performance liquid chromatography; kidney; laboratory rat; malate dehydrogenase; mass spectrometry; matrix assisted laser desorption ionization; membrane channels; nucleic acids; polymerase chain reaction; protein protein interaction; protein structure function; tissue /cell culture

DESCRIPTION (provided by applicant): One of the opportunities to intervene in a disease process is to identify the molecules that participate and then inhibit them. This can be done in theory by identifying the nucleic acids that encode the protein and using a complementary strand of DNA to block this from happening. Unfortunately, while the concept works in solutions of fluid, the ability to do this in a cell has been more difficult. One of the major problems has been understanding how these strands of DNA get into cells. The purpose of this project is to explore this issue. We have previously shown that single stranded DNA can move through a highly specific channel. We purified this protein complex and have been identifying what each component of this complex does. We have now identified one of the subunits of this channel as a common enzyme that serves also to regulate DNA uptake. The current proposal will evaluate other subunits and participants in this activity. We hope to use this information to design DNA molecules as therapeutics to treat disease.

描述（由申请人提供）： 干预疾病过程的机会之一是确定 参与并抑制它们的分子。从理论上可以做到这一点 识别编码蛋白质并使用互补的核酸 DNA链阻止这种情况发生。不幸的是，虽然这个概念 在液体溶液中的作品，在细胞中进行此操作的能力更多 难的。主要问题之一是了解这些链如何 DNA进入细胞。该项目的目的是探索这个问题。我们 以前已经表明，单链DNA可以通过高度移动 特定渠道。我们纯化了这种蛋白质复合物，并且一直在识别 该复合物的每个组成部分的作用。我们现在已经确定了其中一个 该通道的亚基作为一种用于调节DNA的常见酶 吸收。当前的建议将评估其他亚基和参与者 这项活动。我们希望使用这些信息将DNA分子设计为 治疗疾病的治疗剂。