SPECIFIC PATHOGEN FREE RHESUS MACAQUE BREEDING PROGRAM

无特定病原体恒河猴育种计划

• 批准号: 6759265
• 负责人: Greg Westergaard
• 金额: $ 188.93万
• 依托单位: ALPHA GENESIS, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6759265
• 关键词: Macaca mulatta; animal breeding; animal colony; cooperative study; germ free condition

DESCRIPTION: (Adapted from the applicant's abstract): The United States is facing the most critical and devastating epidemic in its recent history resulting from spread of the human immunodeficiency virus (HIV) and its resulting clinical complications, including acquired immune deficiency syndrome (AIDS). Nonhuman primates, phylogenetically our closest living relatives, have always been very important as models for studying human disease, as well as for testing drug therapies and vaccines. The preferred nonhuman primate model for AIDS and AIDS-related studies is the Indian-origin rhesus macaque (Macaca mulatta). It is now possible to conduct basic research on AIDS in these primates with a high degree of confidence that the results will be directly applicable to the human syndrome. Implicit in the development of nonhuman primate disease models is the notion that the recipient model must be completely free of viruses that could potentially compromise research. The National Institutes of Health (NIH) also recognizes that there is a special need for major histocompatibility complex (MHC) defined rhesus macaques for certain types of AIDS-related research. We propose two independent but complementary options through which LABS of Virginia, Inc., a long-term provider of SPF macaques to the biomedical research community, can provide the NIH with large numbers of Indian-origin SPF rhesus macaques and ensure the availability of Indian-origin rhesus macaque breeding stock to meet future research needs. Option #1 provides for purchase and maintenance of an SPF-pedigree breeding colony and up to 260 SPF- pedigree research offspring, and Option #2 provides for purchase and maintenance of a non-SPF breeding colony and up to 412 SPF-derived offspring. SPF is defined as serologically negative and virus culture negative for Herpesvirus simiae, simian immunodeficiency virus (SIV), simian T-lymphotropic virus (STLV-1), and Type D simian retrovirus (SRV: SRV1, SRV2, and SRV5). SPF-pedigree is defined as the offspring of SPF parents and SPF-derived is defined as the offspring of Non-SPF (but seronegative for SRV) parents. Implicit in this objective is management of the existing SPF rhesus macaque resource and development of additional animal resources, including selective breeding of MHC-defined rhesus (up to 140 over a five-year period).

描述：（改编自申请人的摘要）：美国是 面临近代历史上最严重和最具破坏性的流行病 人类免疫缺陷病毒（HIV）及其传播造成的 由此产生的临床并发症，包括获得性免疫缺陷 综合症（艾滋病）。 非人类灵长类动物，在系统发育上与我们最接近的生物 亲属作为研究人类的模型一直非常重要 疾病，以及测试药物疗法和疫苗。 首选 用于艾滋病和艾滋病相关研究的非人类灵长类动物模型起源于印度 恒河猴（Macaca mulatta）。 现在可以进行基础研究 对这些灵长类动物中的艾滋病的研究结果充满信心 将直接适用于人类综合症。 隐含在 非人类灵长类疾病模型的开发是这样的概念： 接受者模型必须完全不含可能存在的病毒 妥协研究。 美国国立卫生研究院 (NIH) 也承认 对主要组织相容性复合体 (MHC) 有特殊需要 定义恒河猴用于某些类型的艾滋病相关研究。 我们 提出两个独立但互补的选项，通过该选项，实验室 Virginia, Inc.，生物医学领域 SPF 猕猴的长期供应商 研究界，可以为 NIH 提供大量印度裔 对恒河猴进行 SPF 保护，确保印度原产恒河猴的供应 猕猴种畜以满足未来的研究需要。 选项 #1 规定 购买和维护 SPF 纯种繁殖群以及最多 260 个 SPF- 谱系研究后代，选项 #2 提供购买和 维持非 SPF 繁殖群体和多达 412 个 SPF 衍生后代。 SPF 被定义为血清学阴性和病毒培养阴性 猴疱疹病毒、猴免疫缺陷病毒 (SIV)、猴 T 淋巴细胞 病毒（STLV-1）和 D 型猿猴逆转录病毒（SRV：SRV1、SRV2 和 SRV5）。 SPF谱系被定义为SPF父母的后代，而SPF衍生的是 定义为非 SPF（但 SRV 血清阴性）父母的后代。 这一目标隐含着对现有 SPF 恒河猴的管理 额外动物资源的资源和开发，包括选择性 繁殖 MHC 定义的恒河猴（五年内最多饲养 140 只）。