MOLECULAR DIAGNOSTIC TESTS FOR SIMIAN HERPESVIRUSES

猿猴疱疹病毒的分子诊断测试

• 批准号: 6951908
• 负责人: R EBERLE
• 金额: $ 25.57万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6951908
• 关键词: Cercopithecid herpesvirus 1; Herpesviridae disease; cell line; communicable disease diagnosis; diagnosis design /evaluation; diagnosis quality /standard; diagnostic tests; early diagnosis; gene expression; gene targeting; genetic strain; glycoproteins; green fluorescent proteins; herpes simplex virus 1; herpes simplex virus 2; immunologic assay /test; laboratory mouse; laboratory rabbit; monoclonal antibody; polymerase chain reaction; reagent /indicator; recombinant proteins; serology /serodiagnosis; virus antigen; virus genetics; virus protein

DESCRIPTION (Provided by applicant): The capability to detect and diagnose infections by non-human primate viruses is far less than optimal and in cases non-existent. As Specific Pathogen Free monkey colonies are developed, it will be essential that maximally sensitive tests for monkey viruses are available to develop and monitor such colonies. The overall goal of the proposed research is to develop basic molecular data for BV of macaques and to utilize these results to develop diagnostic tests that are more sensitive, more specific and safer than those currently available. To achieve these goals, the following experiments will be performed: 1) An indicator cell line will be constructed for diagnostic use in virus isolation which expresses GFP when infected by BV, making virus isolation testing for BV a more sensitive and safer procedure. 2) A lethal-deletion mutant strain of BV (ABV) will be constructed by deleting an essential structural gene and constructing a complementing cell line in which ABV can replicate. This system (ABV + complementing cell line) will make preparation of BV antigens and DNA much safer. 3) Major BV immunogens will be identified by assessing the macaque immune response to individual BV proteins. This information will allow development of effective vaccines and recombinant antigens for use in diagnostic tests. 4) Specific glycoprotein genes of BV will be expressed using an inducible mammalian expression system, and the expressed proteins used to develop immunciassays. 5) A TaqMan assay will be developed that can detect and differentiate herpes simplex virus-1 (HSV I), herpes simplex virus-2 (HSV2) and BV. This will provide an extremely sensitive means of diagnosing human BV cases. Successful completion of the proposed experiments will provide a number of reagents that will allow much safer detection of infectious BV in clinical samples, safer preparation of BV antigen for diagnostic tests, more sensitive and specific serological tests for detection of anti-BV antibodies in both macaque and HSV-positive human sera, and basic information regarding the antigenic determinants of BV which may induce protective immunity against infection.

描述（由申请人提供）：检测和诊断的能力 非人类灵长类动物病毒感染远远远远远远少于最佳感染 不存在。 随着特定无病原体猴子菌落的发展， 对猴子病毒的最大敏感测试至关重要 可用于开发和监测此类殖民地。 总体目标 拟议的研究是开发猕猴BV的基本分子数据和 利用这些结果来开发更敏感的诊断测试， 比当前可用的更具体，更安全。 实现这些 目标，将进行以下实验：1）指示单元线 将构建用于表达GFP的病毒隔离的诊断用途 当被BV感染时，对BV进行病毒隔离测试更加敏感 和更安全的程序。 2）BV（ABV）的致命缺失突变菌株将为 通过删除基本的结构基因并构建一个 补充ABV可以复制的细胞系。 此系统（ABV + 补充细胞系）将使BV抗原和DNA的准备 更安全。 3）将通过评估猕猴来识别主要的BV免疫原子 对单个BV蛋白的免疫反应。 此信息将允许 开发有效的疫苗和重组抗原用于 诊断测试。 4）BV的特异性糖蛋白基因将使用 诱导的哺乳动物表达系统，表达的蛋白质曾经用于 开发免疫。 5）将开发一个可以检测到的Taqman分析 并区分单纯疱疹病毒-1（HSV I），疱疹单纯疱疹病毒-2 （HSV2）和BV。 这将提供一种非常敏感的诊断手段 人类BV病例。 成功完成拟议的实验将 提供许多试剂，可以更安全检测 临床样品中的传染性BV，更安全的BV抗原制备 诊断测试，更敏感和更特定的血清学检测测试 猕猴和HSV阳性人类血清中的抗BV抗体和碱性 有关BV的抗原决定因素的信息，该决定因素可能引起 保护性免疫免受感染。

项目成果

Genetic and functional complementation of the HSV1 UL27 gene and gB glycoprotein by simian alpha-herpesvirus homologs.

猿猴 α-疱疹病毒同源物对 HSV1 UL27 基因和 gB 糖蛋白进行遗传和功能互补。

• DOI: 10.1007/s007050050114
• 发表时间: 1997
• 期刊: Archives of virology
• 影响因子: 2.7
• 作者: Eberle,R;Tanamachi,B;Black,D;Blewett,EL;Ali,M;Openshaw,H;Cantin,EM
• 通讯作者: Cantin,EM

Shedding and transmission of baboon Herpesvirus papio 2 (HVP2) in a breeding colony.

狒狒疱疹病毒狒狒 2 (HVP2) 在繁殖群中的脱落和传播。

• 发表时间: 1998
• 期刊: Laboratory animal science.
• 作者: Eberle,R;Black,DH;Lehenbauer,TW;White,GL
• 通讯作者: White,GL

One-step PCR to distinguish B virus from related primate alphaherpesviruses.

一步 PCR 区分 B 病毒和相关灵长类 α 疱疹病毒。

• DOI: 10.1128/cdli.9.3.716-719.2002
• 发表时间: 2002
• 期刊: Clinical and diagnostic laboratory immunology
• 作者: Hirano,Makoto;Nakamura,Shin;Mitsunaga,Fusako;Okada,Maki;Shirahama,Shuya;Eberle,Richard
• 通讯作者: Eberle,Richard

Efficacy of a B virus gD DNA vaccine for induction of humoral and cellular immune responses in Japanese macaques.

B 病毒 gD DNA 疫苗诱导日本猕猴体液和细胞免疫反应的功效。

• DOI: 10.1016/s0264-410x(02)00175-5
• 发表时间: 2002
• 期刊: Vaccine
• 影响因子: 5.5
• 作者: Hirano,Makoto;Nakamura,Shin;Mitsunaga,Fusako;Okada,Maki;Shimizu,Keiko;Ueda,Masahiro;Bennett,Alice;Eberle,Richard
• 通讯作者: Eberle,Richard

Prevalence of Herpesvirus papio 2 in baboons and identification of immunogenic viral polypeptides.

狒狒中狒狒疱疹病毒 2 型的流行情况及免疫原性病毒多肽的鉴定。

• 发表时间: 1997
• 期刊: Laboratory animal science.
• 作者: Eberle,R;Black,DH;Blewett,EL;White,GL
• 通讯作者: White,GL