CORE--ANIMAL PRODUCTION

核心——畜牧生产

• 批准号: 6563169
• 负责人: TING-KAI K LI
• 金额: $ 13.07万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563169
• 关键词: alcoholic beverage consumption; alcoholic liver cirrhosis; animal breeding; animal colony; behavior test; biological models; biomedical facility; genetic models; genetic strain; inbreeding; laboratory rat; linkage mapping; preference

The Animal Production Core will continue to supply selectively bred and inbred P and NP (alcohol-preferring and -non preferring) rats as well as selectively bred HAD and LAD (high and low alcohol-drinking) rats for experimental use. These rodents will be made available to researchers supported by or affiliated with the ARC at Indiana University, and to collaborating, and independent researchers at other Institutions. In order to accomplish this objective: 1) P and NP lines of rats will continue to be selectively bred by a relaxed selection procedure. 2) P and NP inbred strains, four each, will be maintained by continuing inbreeding. 3) The second and third litters of the selectively bred HAD1/LAD1 and HAD2/LAD2 rat lines from each generation will be produced for research use. 4) Inbreeding of HAD1/LAD1 and HAD2/LAD2 rat lines will be continued. In addition to producing P/NP and HAD/LAD selectively bred and inbred rats, the Animal Production Core will also maintain a colony of HARF/LARF (alcohol-preferring/non preferring rat lines from Toronto based on a limited-access drinking paradigm) rats so that F2s can be raised for Quantitative Trait Loci analysis. Since we recently have characterized the molecular abnormality in the thrombopathia that is seen in NP rats and now have developed inbred P and NP rat strains (brother x sister mating for 22 generations), the Animal Production Core is now in the position to initiate the project to create a congenic NP line that lacks the bleeding disorder. Additionally, experiments will be carried out to determine whether P and HAD rats will develop serious liver disease (i.e., hepatic fibrosis with or without cirrhosis) through voluntary and/or forced ethanol drinking and under conditions that favor alcohol-induced hepatic injury and fibrogenesis.

动物生产核心将继续供应选择性饲养的动物 以及近交系 P 和 NP（酒精偏好和非偏好）大鼠 选择性饲养的 HAD 和 LAD（高饮酒和低饮酒）大鼠 实验使用。 这些啮齿动物将提供给研究人员 由印第安纳大学 ARC 支持或附属，并 其他机构的合作和独立研究人员。为了 为了实现这一目标： 1) 大鼠的 P 和 NP 系将继续 通过宽松的选择程序进行选择性培育。 2) P 和 NP 近交系 每个品系有四个，将通过继续近亲繁殖来维持。 3) 的 选择性繁殖的 HAD1/LAD1 和 HAD2/LAD2 的第二窝和第三窝 每一代的大鼠品系将用于研究用途。 4） HAD1/LAD1 和 HAD2/LAD2 大鼠品系的近亲繁殖将继续进行。 除了产生P/NP和HAD/LAD选择性繁殖和近交 大鼠，动物生产核心还将维持一个 HARF/LARF 群体 （来自多伦多的嗜酒/不嗜酒大鼠品系，基于 限制饮酒模式）大鼠，以便可以饲养 F2 定量性状基因座分析。 由于我们最近已经表征了分子异常 在 NP 大鼠中观察到的血栓病，现在已发展为近交系 P 和 NP 大鼠品系（兄弟 x 姐妹交配 22 代），动物 Production Core 现在可以启动项目来创建 缺乏出血性疾病的同类 NP 系。 此外， 将进行实验以确定 P 和 HAD 大鼠是否会 发展为严重的肝脏疾病（即肝纤维化，伴或不伴 肝硬化）通过自愿和/或强迫饮酒和以下 有利于酒精引起的肝损伤和纤维形成的条件。