Array-microscope slide scanner system

阵列显微镜载玻片扫描仪系统

• 批准号: 6934904
• 负责人: ARTUR G OLSZAK
• 金额: $ 63.53万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-07 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6934904
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; data management; digital imaging; high throughput technology; histopathology; image processing; microscopy; miniature biomedical equipment; optics

DESCRIPTION (PROVIDED BY APPLICANT): We propose the development of an innovative, economical, array-microscope digital slide scanner to generate virtual slides in histopathology. This instrument will be capable of scanning a microscope slide in a single sweep at a numerical aperture of NA = 0.65, covering the entire width and length of a slide with 0.47- um sampling. The fundamental innovation consists in the use of an array of 100 miniaturized objectives, each measuring approximately 2 mm in diameter and 9 mm in length, slightly staggered in the array, to produce a single, seamless sweep of the slide. This innovation allows a very rapid full-color scan requiring less than 60 seconds for a full microscope slide of 20 mm by 50 mu m, at diffraction-limited sampling. This scanning speed is a factor of 10-20 faster than any single-objective system's. The Phase I application laid out the scientific innovation and merit of the array microscope scanning technology. It is the overall goal of Phase II to build on the accomplishments of Phase I and produce an economical, high-throughput digital slide scanner that can be either a stand-alone solution or integrated with existing image-management and analysis solutions. We will achieve this goal through a combination of developments in manufacturing (result: economical scanner) and software (result: tools to seamlessly connect our instrument to third-party laboratory-information-system or quantitative-analysis software). The three Aims of Phase II are: (Aim 1) Develop the means to manufacture high-numerical-aperture array microscope optics at minimal cost. Specifically, implement and perfect injection-molding of high-precision optical elements arranged as arrays on easy-to-assemble, coin-sized plates; (Aim 2) Develop the methods necessary to quality-control (QC) arrays of miniature microscope objectives; and (Aim 3) Develop a Software Development Kit (SDK) to allow third-party developers to create clinical and research solutions that combine DMetrix's array microscope instrument and specialized software.

描述（由申请人提供）：我们建议开发创新，经济，阵列 - 微镜数字幻灯片扫描仪，以生成组织病理学的虚拟幻灯片。该仪器将能够以Na = 0.65的数值孔径在单个扫描中扫描显微镜载玻片，从而覆盖幻灯片的整个宽度和长度，并进行0.47-UM采样。基本创新在于使用100个微型目标的阵列，每个目标的直径约为2 mm，长度为9毫米，在阵列中略微交错，以产生幻灯片的单个无缝扫描。这种创新允许在衍射限制的采样下进行20 mm乘50 mu m的全显微镜载玻片的全彩扫描非常快地需要少于60秒。该扫描速度比任何单目标系统快10-20倍。 第一阶段的应用程序列出了阵列显微镜扫描技术的科学创新和优点。 II阶段的总体目标是建立I阶段的成就，并产生经济的高通量数字幻灯片扫描仪，可以是独立的解决方案，或与现有的图像管理和分析解决方案集成在一起。我们将通过结合制造业（结果：经济扫描仪）和软件（结果：无缝将我们的仪器连接到第三方实验室信息系统或定量 - 分析软件的工具）的结合来实现这一目标。 第二阶段的三个目的是：（目标1）开发一种以最低成本制造高数字孔径显微镜光学器件的手段。具体而言，在易于组装的硬币大小板上排列的高精度光学元件的高精度光学元件的实现和完美注射式销售； （目标2）开发微型显微镜目标质量控制（QC）阵列所需的方法； （AIM 3）开发一个软件开发套件（SDK），以允许第三方开发人员创建结合DMetrix阵列显微镜仪器和专业软件的临床和研究解决方案。