Analysis of MAP kinase cascades in S. cerevisiae

酿酒酵母中 MAP 激酶级联的分析

• 批准号: 6871356
• 负责人: PATRICK J WESTFALL
• 金额: $ 4.99万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6871356
• 关键词: Saccharomyces cerevisiae; biological signal transduction; cell growth regulation; environmental adaptation; environmental stressor; enzyme activity; fungal genetics; gene expression; genetic regulation; histogenesis; microarray technology; mitogen activated protein kinase; nitrogen; osmotic pressure; phosphorylation; postdoctoral investigator; site directed mutagenesis

DESCRIPTION (provided by applicant): Cells often respond to environmental cues by utilizing mitogen-activated protein (MAP) kinase cascades to regulate the expression of genes responsible for the appropriate cellular response. The experiments that I am proposing to conduct seek to advance the understanding of how cells sense environmental stress, activate the appropriate MAP kinase cascade, and then deactivate the cascade once the cell has responded. Nitrogen starvation induces the formation of pseudohyphae in S. cerevisiae via a MAP kinase cascade that shares components with a MAP kinase cascade triggered by high extracellular osmolarity. Studies from the Herskowitz lab have identified Sho1p as a potential sensor for nitrogen starvation in addition to its established role as a sensor of osmolarity. I will carry out genetic screens to identify other proteins involved in sensing low nitrogen. In addition, I shall investigate how Sho1p signaling is regulated to prevent constitutive activation of the high osmolarity glycerol and pseudohyphal growth pathways. Finally, using chemically inhibitable versions of the MAPK Hog1p in conjunction with DNA microarrays, I will identify genes regulated by Hog1p during both stress and non-stress conditions.

描述（由申请人提供）：细胞通常通过利用有丝分裂原激活蛋白（MAP）激酶级联反应来对环境提示做出反应，以调节负责适当细胞反应的基因的表达。我提议进行的实验试图提高对细胞如何感知环境压力的理解，激活适当的MAP激酶级联反应，然后一旦细胞做出响应，将级联反应停用。氮饥饿通过MAP激酶级联反应诱导酿酒酵母中的假性形成，该级数与高细胞外渗透压触发的MAP激酶级联反应共享成分。来自Herskowitz实验室的研究已将SHO1P确定为氮饥饿的潜在传感器，此外它是其作为渗透压传感器的既定作用。我将执行遗传筛选，以鉴定与低氮有关的其他蛋白质。此外，我将研究如何调节SHO1P信号，以防止高渗透压甘油和伪氢生长途径的组成型激活。最后，使用化学可约束的MAPK HOG1P版本与DNA微阵列结合使用，我将在应力和无压力条件下确定由HOG1P调节的基因。