Investigating of a Novel Checkpoint in S. Cerevisiae

酿酒酵母新检查点的研究

• 批准号: 6920712
• 负责人: WENYI FENG
• 金额: $ 4.99万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6920712
• 关键词: DNA replication; DNA replication origin; Saccharomyces cerevisiae; artificial chromosomes; cell cycle; cell cycle proteins; cell morphology; functional /structural genomics; fungal genetics; fungal proteins; gene deletion mutation; genetic mapping; genetic screening; postdoctoral investigator

DESCRIPTION (provided by applicant): Previous work has shown that an origin-deficient YAC (5ORIdeltaYAC) induces a novel checkpoint. This checkpoint is independent of DNA damage. It is also different from the HU-induced replication checkpoint because replication forks do not stall and Rad53 is not activated. Therefore, it was postulated that this novel checkpoint monitors ongoing DNA synthesis. Interestingly, Rad9 was found essential for maintaining this checkpoint, indicating a previously unknown role of Rad9 in monitoring S phase progression in a normal cell cycle. A genetic screen will be conducted to identify additional components of this novel checkpoint pathway. This work will contribute to our understanding of how checkpoint mechanisms operate during an unperturbed cell cycle. It will also further our understanding of how cancer cells escape cell cycle and checkpoint control, leading to genome instability.

描述（由申请人提供）： 先前的工作表明，原始缺陷的YAC（5orideltayac）引起了新的检查点。该检查点与DNA损伤无关。它也与HU诱导的复制检查点不同，因为复制叉不停滞，并且没有激活RAD53。因此，据推测，这个新颖的检查点监测正在进行的DNA合成。 有趣的是，发现RAD9对于维持此检查点至关重要，表明RAD9在监测正常细胞周期中S相位进展中的作用先前未知。将进行遗传筛选，以识别此新型检查点途径的其他组成部分。这项工作将有助于我们理解检查点机制如何在不受干扰的细胞周期中运作。这也将进一步了解癌细胞如何逃脱细胞周期和检查点控制，从而导致基因组不稳定性。