Microenvironmental Regulation of Oval Cell Functions

卵圆细胞功能的微环境调节

基本信息

批准号：
6884513
负责人：
Gregory H Underhill
金额：
$ 4.83万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-07-01 至 2007-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6884513
关键词：
bioengineering /biomedical engineering biological signal transduction biomaterials cell adhesion cell component structure /function cell migration flow cytometry gel integrins liver cells polymerization postdoctoral investigator stem cells tissue engineering

项目摘要

DESCRIPTION (provided by applicant): Hepatic oval cells are an intriguing potential cell source for liver tissue engineering and other cell-based liver therapies due to their ability to be expanded in culture and their bipotential differentiation into hepatocytes or bile duct epithelial cells. However, many aspects of oval cell bjology, in particular, the regulation of oval cell responses by microenvironmental factors, remain undefined. The proposed research will utilize a model system based on photopolymerized hydrogels to investigate oval cell functions in 2-D and 3-D settings. Specifically, these experiments will examine the role of integrins and Notch receptors in specifying oval cell fate processes such as adhesion, migration, and signaling. Photopatterned hydrogel platforms will be utilized to investigate the effect of asymmetric presentation of external cues, and cross-talk between integrins and Notch will also be assessed. These studies will provide fundamental insight into oval cell responses, important for the development of tissue engineered liver therapies. The controlled incorporation of important regulators of oval cell functions in 3-D and/or asymmetrically represents an important advancement towards an oval cell-based tissue engineered liver construct.

描述（由申请人提供）：肝椭圆形细胞是肝组织工程和其他基于细胞的肝脏疗法的吸引人的潜在细胞来源，因为它们在培养物中的能力及其两能力分化为肝细胞或胆管上皮细胞。然而，特别是，椭圆形细胞植物学的许多方面，微环境因素对椭圆形细胞反应的调节仍然不确定。拟议的研究将利用基于光聚合水凝胶的模型系统来研究2-D和3-D设置中的椭圆形细胞功能。具体而言，这些实验将检查整联蛋白和缺口受体在指定椭圆形细胞命运过程中的作用，例如粘附，迁移和信号传导。光图案水凝胶平台将用于研究外部线索不对称表示的影响，并且还将评估整联蛋白和Notch之间的串扰。这些研究将提供对椭圆形细胞反应的基本见解，这对于组织工程肝疗法的发展很重要。椭圆形细胞功能在3-D和/或不对称中受控的调节剂对椭圆基细胞组织工程肝脏构建体的重要进展。