MicroRNA Expression Profiling

MicroRNA 表达谱

• 批准号: 6832899
• 负责人: DAVID M BROWN
• 金额: $ 20.37万
• 依托单位: AMBION, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6832899
• 关键词: RNA; biotechnology; fluorescent dye /probe; functional /structural genomics; gene expression profiling; genetic regulation; genetic transcription; high throughput technology; method development; microarray technology; nucleic acid hybridization; nucleic acid probes; nucleic acid purification; nucleic acid quantitation /detection; oligonucleotides

DESCRIPTION (provided by applicant): MicroRNAs are small, 19-25mer RNAs that are encoded in the genomes of upper eukaryotes. MicroRNAs appear to regulate global gene expression by modulating the translation of mRNAs transcribed from key genes. Although the research field is still young, microRNAs have been implicated in early development, brain development, cell differentiation, apoptosis, and tumor progression. Like mRNA analysis, defining miRNA expression profiles in samples should make it possible to identify miRNAs that are involved in a wide variety of bioogical processes. Towards this end, we propose to create methods for miRNA isolation, labeling, and microarray analysis that will make it possible to catalogue the miRNA profiles of any sample. The procedures will incorporate labeling and array procedures that will allow existing microarray instruments to be used for miRNA analysis.

描述（由申请人提供）：microRNA小，19-25mer rNA，编码在上核生物的基因组中。 microRNA似乎通过调节从关键基因转录的mRNA的翻译来调节全局基因表达。尽管研究领域还很年轻，但MicroRNA与早期发育，脑发育，细胞分化，凋亡和肿瘤进展有关。像mRNA分析一样，定义样品中的miRNA表达谱应该使鉴定参与多种生物学过程的miRNA成为可能。为此，我们建议创建用于miRNA隔离，标记和微阵列分析的方法，以使任何样品的miRNA谱分类成为可能。该过程将结合标记和阵列程序，以允许现有的微阵列仪器用于miRNA分析。