Arrays of Receptors for Infectious Disease Diagnosis

用于传染病诊断的受体阵列

• 批准号: 6744033
• 负责人: Frances S. Ligler
• 金额: $ 60.01万
• 依托单位: U.S. NAVAL RESEARCH LABORATORY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6744033
• 关键词: adhesin; antibiotics; antibody; antigen antibody reaction; bacterial antigens; bacterial toxins; bioengineering /biomedical engineering; biohazard detection; biosensor device; bioterrorism /chemical warfare; communicable disease diagnosis; diagnosis design /evaluation; diagnostic tests; hemoprotein; membrane proteins; microarray technology; oligosaccharides; siderophores

DESCRIPTION (provided by applicant): Goal: Develop an array of capture molecules for pathogens, intended for use in a rapid screening format, that includes the following: 1. specific capture molecules for different species of microorganisms or their toxic products; 2. semi-selective capture molecules that identify pathogens based on mechanisms involved in the process; of pathogenicity; 3. indicators of potential susceptibility to antibiotics. The first step in this process is to modify candidates for the array and immobilize them in a sensor for rapid characterization. The candidates will include antibodies, oligosaccharides, adhesions, siderophores, combinatorial peptides which bind membrane proteins on the pathogen, and antibiotics. The candidates will be exposed to a broad spectrum of pathogens and toxins to determine both specificity and binding affinity. The elements of the array will be selected to provide the widest amount of information possible: species if possible, mechanism of infection or pathogenicity, and antibiotic susceptibility. The array elements will then be transferred to a corporate partner with demonstrated capability of producing thousands of sensor arrays for clinical use.

描述（由申请人提供）：目标：为病原体开发一系列捕获分子，用于快速筛选格式，其中包括以下内容：1。针对不同种类的微生物或其有毒产品的特定捕获分子； 2。基于该过程中涉及的机制鉴定病原体的半选择性捕获分子；致病性； 3。对抗生素敏感的指标。 此过程的第一步是修改阵列的候选物，并将其固定在传感器中以进行快速表征。候选物将包括抗体，寡糖，粘附，铁载体，结合病原体上膜蛋白的组合肽和抗生素。候选人将暴露于多种病原体和毒素中，以确定特异性和结合亲和力。阵列的元素将被选择以提供可能的最广泛的信息：如果可能的话，感染或致病性机理以及抗生素敏感性。然后，阵列元素将被转移到公司合作伙伴中，并具有生产数千个传感器阵列供临床使用的能力。