Olfactory Genes in Mosquitoes

蚊子的嗅觉基因

• 批准号: 6831192
• 负责人: HARALD BIESSMANN
• 金额: $ 35.4万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6831192
• 关键词: Olfactory; Genes; Mosquitoes

EXCEED THE SPACE PROVIDED. To obtain a better understanding of the olfactory processes that allow mosquitoes to identify human hosts, a molecular study has been performed to identify and characterize molecules in the olfactory signaling pathway of the African malaria vector Anopheles gambiae. Using eDNA libraries from antennae of females and males, a collection of cDNAs encoding odorant binding proteins (OBPs) and other antennal proteins has been isolated and characterized. Their spatial and temporal gene expression in A. gambiae antennae using RNA and antibody probes will be determined, and the search for gender-specific expression of antennal genes will be continued. Concentrating on chemicals that have previously been shown to be attractants for female mosquitoes and also elicit an electrophysiological response, in vitro binding assays will be performed to study the interaction of specific odor molecules with mosquito OBP proteins. To investigate if altered expression of specific OBPs in antennae causes detectable behavioral and electrophysiological changes, transgenic mosquitoes will be generated that express RNAi against specific OBPs under the control of strong OBP promoters in their antennae. Suppression of OBP expression will be determined and the modified mosquitoes will be tested in wind tunnel experiments for behavioral changes in response to odorants, and for electrophysiological changes by single sensilla recordings. These studies will ultimately allow the development of better methods to interfere with the mosquito-host interaction and reduce transmission of the malaria parasite. PERFORMANCE SITE ========================================Section End===========================================

超出所提供的空间。 为了更好地了解蚊子识别人类宿主的嗅觉过程，进行了一项分子研究，以识别和表征非洲疟疾媒介冈比亚按蚊嗅觉信号通路中的分子。使用雌性和雄性触角的 eDNA 文库，分离并表征了编码气味结合蛋白 (OBP) 和其他触角蛋白的 cDNA 集合。将使用 RNA 和抗体探针确定它们在冈比亚 A. gambiae 触角中的空间和时间基因表达，并将继续寻找触角基因的性别特异性表达。重点关注先前已被证明对雌性蚊子具有引诱剂并引起电生理反应的化学物质，将进行体外结合测定，以研究特定气味分子与蚊子 OBP 蛋白的相互作用。为了研究触角中特定 OBP 表达的改变是否会导致可检测到的行为和电生理变化，将产生转基因蚊子，在其触角中强 OBP 启动子的控制下表达针对特定 OBP 的 RNAi。将确定 OBP 表达的抑制，并在风洞实验中测试改良蚊子对气味剂的反应行为变化，以及通过单感器记录的电生理变化。这些研究最终将有助于开发出更好的方法来干扰蚊子与宿主的相互作用并减少疟疾寄生虫的传播。表演网站==========================================部分结束====== =======================================