Cellular Localization Of Gadolinium In Cardiac And Vascu

钆在心脏和血管中的细胞定位

• 批准号: 6837259
• 负责人: Richard D Leapman
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6837259
• 关键词: bioimaging /biomedical imaging; blood vessels; cardiovascular imaging /visualization; contrast media; electron probe spectrometry; freezing; gadolinium; heart; image enhancement; intracellular; laboratory rat; myocardial ischemia /hypoxia; scanning transmission electron microscopy; sectioning; spectrometry

The aim of this project is to localize the MRI contrast agent gadolinium in cardiac and vascular tissues using electron probe x-ray microanalysis and electron energy loss spectroscopy (EELS). The sensitivity of the spectroscopic imaging approach is first demonstrated by mapping chelated gadolinium contained in sectioned polymer grains that are used to deliver the contrast agent. Experiments are now being performed to map the distribution of intracellular Gd in ischemic and control rat heart that has been treated with the gadolinium contrast agent. Tissue is rapidly frozen by clamping against liquid nitrogen cooled copper surfaces. Blocks are then transferred to an ultra-cryomicrotome where they are cut to a thickness of approximately 100 nm, picked up on copper grids coated with carbon-coated Formvar, and stored prior to analysis. It is hoped to use this approach to determine which subcellular compartments accumulate gadolinium. The specimens will be analyzed in a high-resolution STEM equipped with an energy-dispersive x-ray spectrometer.

该项目的目的是使用电子探针X射线微分析和电子能量损耗光谱（EELS）将MRI对比剂g gadolin定位在心脏和血管组织中。首先，通过绘制用于传递造影剂的截面聚合物晶粒中包含的螯合gadolinium，首先证明了光谱成像方法的敏感性。现在正在进行实验，以绘制已用gadolinium对比剂治疗的缺血性和对照大鼠心脏中细胞内GD的分布。通过夹紧液氮冷却铜表面来迅速冷冻组织。然后将块转移到超晶状体的超晶状体上，将它们切成约100 nm的厚度，并在涂有碳涂层Formvar的铜网格上拾取，并在分析前存储。希望使用这种方法来确定哪些亚细胞室积累了gadolinium。该样品将在配备能量X射线光谱仪的高分辨率茎中进行分析。