CORE--ELECTROPHYSIOLOGY AND XENOPUS OOCYTE LABORATORY

核心--电生理学和爪蟾卵细胞实验室

• 批准号: 6660036
• 负责人: DAVID C DAWSON
• 金额: $ 17.41万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660036
• 关键词: Xenopus oocyte; animal genetic material tag; biomedical facility; electrophysiology; genetic manipulation

The objective of the Electrophysiology and Xenopus Oocyte is to make readily available to Toxicology Center investigators a variety of electrophysiological assays for ion transport and the Xenopus oocyte expression system for transporters and receptors. The rationale for establishing the core is that these techniques require specialized equipment and/or expertise that might not be available otherwise. The core will offer a broad range of assays ranging from flat-sheet, Ussing Chamber Studies to single channel recording and analysis. In addition the core will be equipped to harvest, defolliculate and inject, with RNA, Xenopus oocytes that can be used as an expression system for transporters or receptors. A primary aim of the Center for Membrane Toxicity Studies is to investigate the mechanism for the toxic effects of metals on membrane proteins, transporters and receptors. The Electrophysiology and Xenopus Oocyte Core will provide the equipment and expertise for Center investigators who wish to employ these important technologies in their research.

电生理学和非洲爪蟾卵母细胞的目标是使 毒理学中心研究人员可以随时获取各种 离子转运和非洲爪蟾卵母细胞的电生理测定 转运蛋白和受体的表达系统。 理由 建立这些技术的核心是需要专门的 否则可能无法获得的设备和/或专业知识。 这 core 将提供广泛的检测，包括平板、Ussing 单通道录音和分析的室内研究。 此外 核心将配备用于收获、去毛囊和注射 RNA， 可用作转运蛋白表达系统的非洲爪蟾卵母细胞 或受体。 膜毒性研究中心的主要目标 目的是研究金属对人体的毒性作用的机制。 膜蛋白、转运蛋白和受体。 电生理学和 非洲爪蟾卵母细胞核心将为中心提供设备和专业知识 希望在他们的研究中使用这些重要技术的研究人员 研究。