Roles of Recoding and Transcription Slippage

重新编码和转录滑动的作用

• 批准号: 6817478
• 负责人: RAYMOND F GESTELAND
• 金额: $ 29.9万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6817478
• 关键词: DNA directed RNA polymerase; SDS polyacrylamide gel electrophoresis; Saccharomyces cerevisiae; comparative genomic hybridization; frameshift mutation; gene expression; genetic transcription; green fluorescent proteins; human genetic material tag; mass spectrometry; messenger RNA; molecular genetics; molecular site; nucleic acid sequence; open reading frames; polymerase chain reaction; protein biosynthesis

DESCRIPTION (provided by applicant): The expression of a minority of genes involves a substantial proportion of ribosomes shifting reading frame at specific site(s). This frameshifting occurs at shift-prone sites and is commonly greatly stimulated by signals embedded elsewhere in the mRNA, often nearby; i.e. it is programmed. It is proposed to computationally identify candidates for utilized frameshifting in vertebrates especially mammals, and experimentally test them. The goal is to determine the roles of this type of recoding in mammals. Another type of recoding is the dynamic programming of stop codons. Cases where the redefinition involves insertion of a standard amino acid, rather than selenocysteine, will also be similarly investigated. Finally, the extent and function of slippage of RNA polymerase slippage at runs of repeat bases will be assessed. Some occurrences of translational and transcriptional slippage-prone sequences will not be utilized for gene expression and their consequences will be solely errors. The extent of these errors will be investigated.

描述（由申请人提供）：少数基因的表达涉及相当大比例的核糖体在特定位点移动阅读框。这种移码发生在易发生移码的位点，并且通常会受到嵌入在 mRNA 其他位置（通常在附近）的信号的极大刺激；即它已被编程。建议通过计算确定在脊椎动物尤其是哺乳动物中使用移码的候选者，并进行实验测试它们。目标是确定这种类型的重新编码在哺乳动物中的作用。另一种类型的重新编码是终止密码子的动态编程。重新定义涉及插入标准氨基酸而不是硒代半胱氨酸的情况也将进行类似的研究。最后，将评估重复碱基运行时 RNA 聚合酶滑移的程度和功能。一些出现的翻译和转录易滑移序列将不会被用于基因表达，其后果将仅仅是错误。将调查这些错误的程度。