Different Notch Receptors in Drosophila Development

果蝇发育中的不同Notch受体

• 批准号: 6766865
• 负责人: CEDRIC S WESLEY
• 金额: $ 22.73万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6766865
• 关键词: Drosophilidae; arthropod genetics; biological signal transduction; cell differentiation; developmental genetics; developmental neurobiology; protein binding; receptor expression

DESCRIPTION (provided by applicant): Notch receptors generate different types of differentiation signals during development of humans. Accordingly, their loss or aberrant activities result in different types of developmental defects and diseases. To understand the basis for these maladies, and be able to deftly deal with each one of them, we must understand all the different signals generated by these receptors. Drosophila offers an ideal system for the purpose. During differentiation of the central nervous system and epidermis in Drosophila embryos, Notch generates four to five different types of signals. The signals generated to specify the epidermal precursor cells are well understood. However, very little is known about the other types of Notch signals, the intracellular pathways transducing them, or the mechanism by which they are generated. Recent studies show that the full-length Notch receptor, the primary receptor, generates two secondary receptors showing different activities than the primary receptor. The different activities of the three receptors together have the potential to account for all the different types of differentiation signals generated by Notch. The proposed studies will use molecular genetic and biochemical analyses to determine the following. (1) The nature of in vivo signals generated by the different Notch receptors at different stages of differentiation. (2) Whether or not signals from the secondary receptors are transduced by the same pathway that transduces the epidermal precursor cell signals. (3) Whether proteins known to bind Notch regulate the production and activities of the different receptors. These studies are expected to lead to an understanding of how the primary and secondary Notch receptors regulate differentiation of tissues during development.

描述（由申请人提供）：Notch受体生成不同类型 人类发展过程中的分化信号。因此，他们 损失或异常活动导致不同类型的发育缺陷 和疾病。了解这些疾病的基础，并能够巧妙 与每个人打交道，我们必须了解所有不同的信号 由这些受体产生。果蝇为 目的。在区分中枢神经系统和表皮期间 果蝇胚胎，Notch会产生四到五种不同类型的信号。 为指定表皮前体细胞而生成的信号很好 理解。但是，对其他类型的缺口知之甚少 信号，细胞内途径转导的信号，或者 它们是生成的。最近的研究表明，全长缺口受体， 主要受体产生两个显示不同的二级受体 活动比主要受体。这三个活动的不同活动 受体在一起有可能考虑所有不同类型的 Notch产生的分化信号。拟议的研究将使用 分子遗传和生化分析以确定以下内容。 （1） 由不同的Notch受体生成的体内信号的性质 分化的不同阶段。 （2）是否来自 次级受体被相同的途径转导 表皮前体细胞信号。 （3）是否已知可以结合缺陷的蛋白质 调节不同受体的生产和活动。这些 预计研究将导致对主要和如何 次级缺口受体调节组织的分化 发展。