Development of the Precerebellar System

小脑前系统的发育

• 批准号: 6622367
• 负责人: CATHERINE S. BRANDA
• 金额: $ 4.16万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6622367
• 关键词: cell migration; cerebellum; chick embryo; developmental neurobiology; gene expression; genetic techniques; laboratory mouse; neurogenesis; predoctoral investigator

DESCRIPTION (provided by applicant): Critical to cerebellar function is a set of five brainstem neuron assemblies (nuclei) collectively referred to as the precerebellar system. These nuclei are responsible for relaying and processing information that flows from the cerebral cortex and spinal cord to the cerebellum. Precerebellar neurons are generated from dorsal neuroepithelium (the rhombic lip) yet settle in ventral aspects of the medulla and pontine brainstem. To reach their final positions, these neurons must migrate long distances across several brain subdivisions filled with densely packed, synaptically-connected neuropile. Thus, research addressing the development of this crucial, yet rarely studied, neural complex should further elucidate mechanisms underlying cell migration in the mature brain, as well as contribute to our understanding of a critical aspect of cerebellar function. Using clonal analysis, serial analysis of gene expression, and recombinase based fate mapping and mutagenesis techniques, the proposed project will focus on the mechanisms by which precerebellar neuronal identity is established within the rhombic lip.

描述（由申请人提供）：对小脑功能至关重要的是一组 五个脑干神经元组件（核）统称为 小脑前系统。这些核负责中继和处理 信息从大脑皮层和脊髓流向 小脑。小脑前神经元由背侧神经上皮产生 （菱形唇）仍位于延髓和脑桥的腹侧 脑干。为了到达最终位置，这些神经元必须迁移很长一段时间 跨越几个大脑分区的距离，充满了密集的、 突触连接的神经丛。因此，针对发展的研究 这个至关重要但很少研究的神经复合体应该进一步阐明 成熟大脑中细胞迁移的机制，并有助于 帮助我们理解小脑功能的一个关键方面。使用克隆 分析、基因表达系列分析和基于重组酶的命运 作图和诱变技术，拟议项目将重点关注 小脑前神经元身份在大脑中建立的机制 菱形唇。