Chemical Approaches to the Study of Cell Division

研究细胞分裂的化学方法

• 批准号: 6649050
• 负责人: Gregory P Tochtrop
• 金额: $ 4.16万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6649050
• 关键词: SDS polyacrylamide gel electrophoresis; Xenopus oocyte; affinity chromatography; analytical chemistry; biochemistry; cell cycle; cell growth regulation; chemical genetics; chemical structure function; chemical synthesis; cyclins; ion exchange chromatography; method development; postdoctoral investigator; protein transport; proteolysis; western blottings

DESCRIPTION (provided by applicant): The broader goals of the proposed work are aimed at the development of chemical approaches to the study of cell division and ultimately cancer. More specifically, the developing field of 'chemical genetics' will be applied to the understanding of cell cycle regulators and the mitotic spindle checkpoint. The King laboratory has identified several compounds that block exit from mitosis and inhibit cyclin proteolysis. The goals of the proposed research are to identify the biochemical targets of these compounds and pursue the mechanism of how these compounds inhibit cyclin proteolysis. Additionally, we propose to develop a new strategy that will enable the rapid identification of protein targets of active compounds found in chemical genetic screens.

描述（由申请人提供）： 拟议工作的更广泛的目标旨在开发细胞分裂研究和最终癌症的化学方法。更具体地说，“化学遗传学”的发展场将应用于对细胞周期调节剂和有丝分裂主轴检查点的理解。国王实验室已经确定了几种堵塞有丝分裂并抑制细胞周期蛋白蛋白水解的化合物。拟议研究的目标是确定这些化合物的生化靶标，并追求这些化合物如何抑制细胞周期蛋白蛋白水解的机制。此外，我们建议制定一种新的策略，该策略将能够快速鉴定化学遗传筛选中活性化合物的蛋白质靶标。