Analysis of a Shigella dysenteriae Fe regulated promoter

痢疾志贺氏菌 Fe 调控启动子的分析

• 批准号: 6693603
• 负责人: ERIN R MURPHY
• 金额: $ 4.16万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6693603
• 关键词: Shigella dysenteriae; bacterial genetics; flow cytometry; gene expression; genetic promoter element; heme; iron; microarray technology; polymerase chain reaction; postdoctoral investigator; tissue /cell culture

DESCRIPTION (provided by applicant): Millions of cases of shigellosis, caused by the Shigella species, are reported annually. Shigella spp. must acquire all essential nutrients, including iron (Fe), to survive and cause disease within the human host. The presence of Fe within the microenvironment influences the expression of genes whose products are required for Fe acquisition by Shigella (Mills and Payne, 1995; Mills and Payne, 1997). The mechanisms governing Fe regulated gene expression are not fully understood. This proposal aims to characterize the mechanisms controlling the Fe influenced expression of shuA, a gene encoding an outer membrane heme receptor whose function is essential for the utilization of heme as a source of nutrient Fe by S. dysenteriae. The specific aims set forth in this proposal are to: 1) characterize the promoter controlling expression of S. dysenteriae shuA; 2) investigate the mechanisms controlling the Fe-regulated expression of shuA; and 3) analyze the influence of heme on the expression of shuA. A complete understanding of Fe acquisition, including the genes and proteins involved, will lead to a fuller appreciation of the strategies employed by Shigella spp. for survival and proliferation within the human host.

描述（由申请人提供）：每年报告数百万例由志贺氏菌引起的志贺氏菌病病例。志贺氏菌属必须获取所有必需的营养物质，包括铁 (Fe)，才能在人类宿主体内生存并引起疾病。 微环境中铁的存在会影响志贺氏菌获取铁所需的基因表达（Mills 和 Payne，1995；Mills 和 Payne，1997）。 Fe 调节基因表达的机制尚不完全清楚。本提案旨在表征控制 Fe 影响 shuA 表达的机制，shuA 是编码外膜血红素受体的基因，其功能对于痢疾沙门氏菌利用血红素作为营养物 Fe 来源至关重要。 本提案提出的具体目标是： 1) 表征控制痢疾沙门氏菌 shuA 表达的启动子； 2) 研究铁调控shuA表达的机制； 3)分析血红素对shuA表达的影响。 对 Fe 获取的完整了解，包括所涉及的基因和蛋白质，将有助于更全面地了解志贺氏菌属所采用的策略。为了在人类宿主内生存和增殖。