Core--Protein Facility

核心--蛋白质设施

• 批准号: 6800915
• 负责人: JAIME PASCUAL
• 金额: $ 25.53万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6800915
• 关键词: Bacillus anthracis; Clostridium botulinum; bacterial proteins; biomedical facility; bioterrorism /chemical warfare; calorimetry; cell death; circular dichroism; fluorimetry; molecular cloning; protein purification; protein quantitation /detection; surface plasmon resonance; virulence

The emphasis of the expression Core is on providing a uniform set of procedures for protein expression, protein folding and preliminary structural studies, and to provide expertise for measuring protein-protein interactions using a range of equipment available at the Burnham Institute. Included in the scope of this Core are: 1. State-of-the-art protein expression and purification of virulence factors and their host cell targets using high-throughput technologies 2. Large scale purification of isotopically labeled proteins for NMR 3. Preliminary screens for crystallization, diffraction quality and NMR studies. 4. Analysis of protein folding and inhibitor/protein interactions, using SPR, DSC, CD, ITC, stopped-flow fluorimetry. The Expression Core will be utilized for the production of quantities of pure proteins suitable for 3-D structural analysis. In addition, Projects will require substantial use of the Core to generate protein targets for high throughput combinatorial library screening.

Core 表达的重点是为蛋白质提供一套统一的程序 表达、蛋白质折叠和初步结构研究，并提供专业知识 使用伯纳姆提供的一系列设备测量蛋白质-蛋白质相互作用 研究所。该核心范围包括： 1. 最先进的毒力因子及其宿主细胞的蛋白质表达和纯化 使用高通量技术的目标 2. 用于 NMR 的同位素标记蛋白质的大规模纯化 3. 结晶、衍射质量和 NMR 研究的初步筛选。 4. 使用 SPR、DSC、CD、ITC 分析蛋白质折叠和抑制剂/蛋白质相互作用， 停流荧光测定法。 表达核心将用于生产大量适合 3-D 结构分析的纯蛋白质。此外，项目将需要大量使用 Core 来生成用于高通量组合文库筛选的蛋白质靶标。