New Immuno-Active Compound for Optimizing Echinacea

用于优化紫锥菊的新型免疫活性化合物

• 批准号: 6813325
• 负责人: DAVID S PASCO
• 金额: $ 28.22万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6813325
• 关键词: alternative medicine; biotherapeutic agent; chemical structure; gas chromatography mass spectrometry; immunomodulators; ion exchange chromatography; leukocyte activation /transformation; melanins; method development; monocyte; plant extracts; species difference

DESCRIPTION (provided by applicant): Echinacea species represent one of the most widely used immunostimulatory botanicals in the United States herbal market. These products vary considerably with respect to the species used, the plant part, and the formulation. Since these different products all have distinctly different chemistries, it should not be surprising that they would exhibit different pharmacological effects. Formulation variability and therefore chemical variability contributes to the problem in interpreting clinical trials performed to date on these different preparations. It also underscores the importance of identifying the clinically relevant compounds within the different preparations so that standardized consistent products could be produced for the consumer market and for use in clinical trials. Work under our NCAAM funded R21 proposal uncovered a previously unrecognized and potent, monocyte-activating compound, that is present in high concentrations (5% of dry plant weight). A reproducible method for its extraction revealed that its activity can vary as much as 1,000 times among different plant parts of the commercially relevant Echinacea species. The main reason this potent immunostimulatory component has eluded detection is its limited solubility in solvents normally used in natural product chemistry laboratories. Our long-term working hypothesis is that this compound is a major contributor to the immune enhancing properties of this herb and together with the activities contributed by other components produce the overall therapeutic effectiveness of Echinacea. The goal of this project is to provide the means to more accurately standardize Echinacea material using this potent immunostimulatory compound so that more defined products can be used for animal research and clinical trials. It could more directly influence human health by providing information on the types of Echinacea products that contain substantial amounts of this compound (i.e. tinctures, pressed juice, plant part or species). The objectives are: 1. Determine the structural characteristics of this new compound important for activity and develop a standardization procedure 2. Evaluate the variability of this activity in commercial Echinacea plant material and preparations. 3. Investigate agronomic practices that might influence the levels and activity of this compound.

描述（由申请人提供）：紫锥菊物种代表了美国草药市场中使用最广泛的免疫刺激性植物药之一。这些产品在所用物种，植物部分和配方方面有很大不同。由于这些不同的产品都具有明显不同的化学作用，因此它们会表现出不同的药理作用，这不足为奇。配方变异性以及化学变异性有助于解释迄今为止在这些不同的制剂中进行的临床试验的问题。它还强调了在不同制剂中识别临床相关化合物的重要性，以便可以为消费市场生产标准化的一致产品并用于临床试验。 我们在NCAAM资助的R21提案下的工作发现了先前未识别的，有效的单核细胞激活化合物，该化合物的浓度高（占干植物重量的5％）。一种可再现的提取方法表明，其活性在商业相关的紫锥菊物种的不同植物部分中的变化多达1000倍。这种有效的免疫刺激成分被发现检测到的主要原因是在通常用于天然产品化学实验室通常使用的溶剂中溶解度有限。我们的长期工作假设是，这种化合物是该草药的免疫增强特性的主要贡献者，以及其他成分贡献的活动产生了紫锥菊的整体治疗有效性。该项目的目的是使用这种有效的免疫刺激化合物来提供更准确地标准化紫锥菊材料的手段，以便可以将更多定义的产品用于动物研究和临床试验。它可以通过提供有关包含大量这种化合物的紫锥菊产品类型的信息（即tin剂，压榨果汁，植物部分或物种），从而更直接地影响人类健康。目标是： 1。确定这种新化合物的结构特征对于活动和制定标准化程序很重要 2。评估商业紫锥菊植物材料和制剂中这种活动的可变性。 3.研究可能影响该化合物水平和活性的农艺实践。