MICROSCOPIC MRI T2 ANISOTROPY IN ARTICULAR CARTILAGE

关节软骨的显微 MRI T2 各向异性

• 批准号: 6497437
• 负责人: YANG XIA
• 金额: $ 14.77万
• 依托单位: OAKLAND UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497437
• 关键词: animal tissue; articular cartilage; bioimaging /biomedical imaging; biomarker; biomechanics; biophysics; collagen; electron microscopy; histology; intermolecular interaction; light microscopy; magnetic resonance imaging; microscopy; musculoskeletal disorder diagnosis; noninvasive diagnosis; osteoarthritis; protein structure function; proteoglycan; statistics /biometry; water solution

A lack of non-invasive markers to detect cartilage degradation has prevented a fundamental understanding of the development of osteoarthritis (OA), as well as early diagnosis of and intervention in OA. Unlike convention Magnetic Resonance Imaging (MRI) that measures or compares only cartilage image intensity, area or volume, Microscopic MRI (muMRI) is well-suited for the quantitative study of articular cartilage. The muMRI can produce quantitative images not only of water distribution, but also of unique parameters reflecting molecular level information in the tissue at microscopic resolution. In a recent muMRI study using the spin-spin relaxation time (T2), we demonstrated quantitatively at 14muM pixel resolution that (1) there is a unique T2 anisotropy in canine articular cartilage. Our goal is to use T2 relaxation as a sensitive and non-invasive MRI marker to study the tissue structure and molecular interactions in articular cartilage. We propose a number of specific aims (1) to determine the relationship between the T2 anisotropy by muMRI and the histological orientation of the collagen fibrils; (2) to study the heterogeneity of the T2 anisotropy at different load-bearing areas in a joint; (3) to detect the localized anisotropies of the T2 distribution profile in cartilage; (4) to determine the role of the proteoglycan component of cartilage on the T2 anisotropy; (5) to compare the T2 characteristics of normal cartilage with that of naturally lesioned cartilage; and (6) to determine the zone-specific characteristics in cartilage during loading. In combination, these six aims will yield otherwise unavailable new information to elucidate various biophysical, biochemical and biological mechanisms and properties in this important biomaterial at the molecular level. Our proposal is unique in combining the high-resolution nature of muMRI, the quantitative T2 measurement, and histological information. The perfection of the muMRI techniques is the greatest strength of our work. We can now examine the tissue properties in individual histological zones in cartilage non-invasively and at microscopic resolution, thus contributing to the understanding, and ultimately, prevention of arthritic disease.

缺乏检测软骨退化的非侵入性标记物 阻碍了对发展的基本认识 骨关节炎（OA），以及早期诊断和干预 办公自动化。与测量或测量的传统磁共振成像 (MRI) 不同 仅比较软骨图像强度、面积或体积、显微 MRI (muMRI) 非常适合关节的定量研究 软骨。 muMRI 不仅可以生成水的定量图像 分布，而且具有反映分子水平的独特参数 组织中的微观分辨率信息。 在最近一项使用自旋-自旋弛豫时间 (T2) 的 muMRI 研究中，我们 在 14μM 像素分辨率下定量证明 (1) 犬关节软骨独特的 T2 各向异性。我们的目标是使用 T2 弛豫作为一种敏感且非侵入性 MRI 标记来研究 关节软骨的组织结构和分子相互作用。我们 提出若干具体目标（1）确定关系 muMRI 的 T2 各向异性与组织学方向之间的关系 胶原纤维； (2)研究T2的异质性 接头中不同承载区域的各向异性； (3) 检测 软骨中 T2 分布轮廓的局部各向异性； (4) 确定软骨的蛋白多糖成分对软骨的作用 T2各向异性； (5) 正常软骨的T2特征比较 与自然受损的软骨； (6) 确定 加载过程中软骨的区域特定特征。在 结合起来，这六个目标将产生原本无法获得的新目标 阐明各种生物物理、生物化学和生物学的信息 这种重要生物材料的分子机制和特性 等级。 我们的建议是独特的，结合了 muMRI 的高分辨率特性， 定量 T2 测量和组织学信息。这 muMRI 技术的完善是我们工作的最大优势。 我们现在可以检查个体组织学中的组织特性 以非侵入性和微观分辨率对软骨区域进行分析，因此 有助于理解并最终预防 关节炎疾病。