The role of Ena/VASP proteins in muscle development

Ena/VASP 蛋白在肌肉发育中的作用

• 批准号: 6789767
• 负责人: KATHERINE A KRAGTORP
• 金额: $ 4.11万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-17 至 2007-05-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6789767
• 关键词: Xenopus; actin binding protein; actins; axon; cell differentiation; cell motility; confocal scanning microscopy; histogenesis; immunocytochemistry; in situ hybridization; muscle cells; myogenesis; myotubes; oligonucleotides; polymerase chain reaction; postdoctoral investigator; protein structure function; sarcomeres; striated muscles

DESCRIPTION (provided by applicant): The role of Ena/VASP proteins in the organization of the actin cytoskeleton and the regulation of cell motility and axon outgrowth has been well established. However, their expression in embryonic muscle and muscle precursors suggests an additional role in the organization and differentiation of muscles during development. I propose to examine the spatial and temporal expression, and the subcellular localization of Ena/VASP proteins during muscle development, using both Xenopus embryos and cultured primary chick skeletal muscle cells. Construction and expression of deletion mutants of these proteins will allow determination of the domains necessary for targeting to specific subcellular Iocalizations, and provide insights into the molecular basis of Ena/VASP function in the myotome. Using a dominant negative construct and morpholino oligonucleotides to specifically inhibit Ena/VASP protein function and expression, I will investigate the effects of this loss on cytoskeletal and cellular structure and function during development. These investigations will elucidate the role of Ena/VASP proteins in skeletal muscle development, and may produce novel information concerning the molecular and cellular basis of muscle adaptation and disease.

描述（由申请人提供）：ENA/VASP蛋白在肌动蛋白细胞骨架组织中的作用以及细胞运动和轴突生长的调节。但是，它们在胚胎肌肉和肌肉前体中的表达表明在发育过程中的组织和肌肉的分化中发挥了额外的作用。 我建议在肌肉发育过程中使用Xenopus胚胎和培养的原代小鸡骨骼肌细胞来检查肌肉发育过程中ENA/VASP蛋白的亚细胞定位。这些蛋白质的缺失突变体的构建和表达将允许确定靶向特定亚细胞体积化所必需的域，并提供对肌动物中ENA/VASP功能的分子基础的见解。使用主要的负构建体和吗啡寡核苷酸来特别抑制ENA/VASP蛋白功能和表达，我将研究这种损失对发育过程中细胞骨架和细胞结构以及功能的影响。这些研究将阐明ENA/VASP蛋白在骨骼肌发育中的作用，并可能产生有关肌肉适应和疾病的分子和细胞基础的新信息。